COMPARISON OF DIRECT AGGLUTINATION TEST (DAT), INDIRECT IMMUNOFLUORESCENT ANTIBODY (IF AT) AND ELISA IN DIAGNOSIS OF VISCERAL LEISHMANIASIS

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MAHAMI M.; MOHEBALI M.; KESHAVARZ H.; ZAREEI Z.A.

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Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2008 | Volume:8 | Issue:1 (27) Page(s): 77-83

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Title

COMPARISON OF DIRECT AGGLUTINATION TEST (DAT), INDIRECT IMMUNOFLUORESCENT ANTIBODY (IF AT) AND ELISA IN DIAGNOSIS OF VISCERAL LEISHMANIASIS

Author(s)

MAHAMI M. | MOHEBALI M. | KESHAVARZ H. | ZAREEI Z.A. | Issue Writer Certificate

Keywords

VISCERAL LEISHMANIASIS (KALA-AZAR)Q3

OATQ3

IFATQ3

ELISAQ3

Abstract

Background & Objective: Visceral leishmaniasis (kala-azar) is an infectious-parasitic disease occurring sporadically in various parts of Iran. The disease is endemic in Ardabil, East Azarbaijan, Bushehr and Fars provinces. This study was performed with the aim of evaluation and comparison of direct agglutination test (OAT), indirect immunofluorescent antibody (IFAT) and ELISA in sero-diagnosis of visceral leishmaniasis that is following a seroepidemiological survey in Germi district of Ardabil province.Methods: Cluster sampling has been performed from£12 years old and 10% of the adult population in Germi district. Altogether 1155 blood specimens were collected to detect anti- Leishmania antibodies. The samples were tested by direct agglutination test (OAT), indirect immunofluorescent antibody (IFAT) and ELISA methods.Results: 32 (2.8%) of the collected specimens have anti-Leishmania antibodies with titers ³1:800 and from total specimens 7 (0.6%) were positive with ³1:3200 titers. In IFA test 32 (2.8%) have titers ³1:40 and from total specimens 6 (0.52%) were positive with ³1:320 titers. In ELISA test 8 specimens were positive and other specimens were negative.Conclusions: According to the results of this study, IFAT and ELISA are specific and high sensitive tests for sero-diagnosis of visceral leishmaniasis but these methods are expensive and require the complicated tools. Whereas, OAT is a simple method, not expensive and require a simple local laboratory with one or two trained technicians. Therefore OAT could be used for seroepidemiological studies and sero-diagnosis of visceral leishmaniasis in endemic areas instead of IFA and ELISA methods.

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APA: Copy

MAHAMI, M., MOHEBALI, M., KESHAVARZ, H., & ZAREEI, Z.A.. (2008). COMPARISON OF DIRECT AGGLUTINATION TEST (DAT), INDIRECT IMMUNOFLUORESCENT ANTIBODY (IF AT) AND ELISA IN DIAGNOSIS OF VISCERAL LEISHMANIASIS. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 8(1 (27)), 77-83. SID. https://sid.ir/paper/59861/en

Vancouver: Copy

MAHAMI M., MOHEBALI M., KESHAVARZ H., ZAREEI Z.A.. COMPARISON OF DIRECT AGGLUTINATION TEST (DAT), INDIRECT IMMUNOFLUORESCENT ANTIBODY (IF AT) AND ELISA IN DIAGNOSIS OF VISCERAL LEISHMANIASIS. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2008;8(1 (27)):77-83. Available from: https://sid.ir/paper/59861/en

IEEE: Copy

M. MAHAMI, M. MOHEBALI, H. KESHAVARZ, and Z.A. ZAREEI, “COMPARISON OF DIRECT AGGLUTINATION TEST (DAT), INDIRECT IMMUNOFLUORESCENT ANTIBODY (IF AT) AND ELISA IN DIAGNOSIS OF VISCERAL LEISHMANIASIS,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 8, no. 1 (27), pp. 77–83, 2008, [Online]. Available: https://sid.ir/paper/59861/en

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