PRODUCTION AND QUALITY CONTROL OF 153SM-DOTA –AVASTIN

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YAVARI K.

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Journal Paper

Paper Information

Journal: Journal of Ardabil University of Medical Sciences Year:2012 | Volume:11 | Issue:4 (42) Page(s): 380-389

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Title

PRODUCTION AND QUALITY CONTROL OF 153SM-DOTA –AVASTIN

Author(s)

YAVARI K. | Issue Writer Certificate

Keywords

RADIOIMMUNOTHERAPYQ2

AVASTINQ2

153SMQ2

DOTA-NHSQ2

Abstract

Background & Objectives: Antibody-based radiopharmaceutical drugs are the great current interest in imaging and radiotherapy of cancers, and provide an important tool for target specific delivery of radionuclides to specific antigens in the diseased tissues. The monoclonal antibody AVASTIN binds, neutralizes VEGF (Vascular endothelial growth factor) and blocks VEGF-induced angiogenesis in tumor tissues. In this study, the complex of AVASTIN and beta particle was investigated as a first step in the production of a radiopharmaceutical drug.Methods: Antibody of AVASTIN was prepared and purified. The antibody was conjugated with freshly prepared DOTA-NHS and then labeled with 153Sm-samarium chloride (185 MBq). The efficiency and in vitro stability of antibody labeling were determined using thin layer chromatography. The integrity of the radiolabeled antibody was checked by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) protocol. Biodistribution study of 153Sm-DOTA –Avastin was performed in BALB/c mice at 2, 24, 48 and 72 hours after injection.Results: The efficiency of antibody labeling was more than 98%. The in vitro stability of the labeled product in human serum after 120h was 83±2%. There was no fragmentation in the labeled antibody during SDS-PAGE protocol. The highest (%ID/g) was observed in the liver, lungs and kidneys.Conclusion: The monoclonal antibody AVASTIN against angiogenesis was effectively radiolabeled with 153Sm. The Biodistribution study showed that it has a high specificity to accumulation in tissues with enriched blood vessels.

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APA: Copy

YAVARI, K.. (2012). PRODUCTION AND QUALITY CONTROL OF ¹⁵³SM-DOTA –AVASTIN. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 11(4 (42)), 380-389. SID. https://sid.ir/paper/59986/en

Vancouver: Copy

YAVARI K.. PRODUCTION AND QUALITY CONTROL OF ¹⁵³SM-DOTA –AVASTIN. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2012;11(4 (42)):380-389. Available from: https://sid.ir/paper/59986/en

IEEE: Copy

K. YAVARI, “PRODUCTION AND QUALITY CONTROL OF ¹⁵³SM-DOTA –AVASTIN,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 11, no. 4 (42), pp. 380–389, 2012, [Online]. Available: https://sid.ir/paper/59986/en

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