EFFECT OF QRDR MUTATIONS ON CIPROFLOXACIN RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NOURI R.; AHANGARZADEH REZAEE M.; HASANI A.; SAGHAZADEH M.; ASGHARZADEH M.; GHOJAZADEH M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2016 | Volume:16 | Issue:2 Page(s): 116-123

Download Full-Text

Persian Verion

View:

1,087

Download:

Cites:

Information Journal Paper

Title

EFFECT OF QRDR MUTATIONS ON CIPROFLOXACIN RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

Author(s)

Keywords

PSEUDOMONAS AERUGINOSAQ2

FLUOROQUINOLONEQ2

ANTIBIOTIC RESISTANCEQ2

QRDRQ1

Abstract

Background & objectives: FLUOROQUINOLONEs have important role in treatment of P. aeruginosa infections. The main mechanism of FLUOROQUINOLONEs resistance in P. aeruginosa is mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes.The aim of this study was to investigate the role of these mutations in ciprofloxacin resistance in different clinical isolates of P. aeruginosa.Methods: A total of 75 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz. Minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated by Etest assay. DNA sequences of the QRDR of gyrA and parC were determined by dideoxy chain termination method.Results: From 75 isolates, 77.33% were resistant to ciprofloxacin. No amino acid changes were detected in gyrA or parC genes of the ciprofloxacin susceptible isolates. Thr-83→Ile substitution in gyrA was observed in all ciprofloxacin resistant isolates. About 90% of them had Ser-87→Leu substitution in parC. Geometric mean MICs of ciprofloxacin were different for various clinical isolates of P. aeruginosa which had the same situation in type and location of gyrA and parC mutations. Moreover, the geometric mean MIC in isolates from urine was significantly (p<0.05) higher than isolates from tracheal aspirates.Conclusion: Mutations in gyrA and parC genes are the major mechanisms for ciprofloxacin resistance in clinical isolates of P. aeruginosa. Moreover, the role of different effective factors in FLUOROQUINOLONE resistance can be different in various clinical isolates of P. aeruginosa.

Cites

MUTATIONS OF GYRA AND PARC GENES IN CIPROFLOXACIN-RESISTANCE ISOLATES OF PSEUDOMONAS AERUGINOSA FROM BURN WOUNDS IN YAZD CITY, IRAN

References

Cite

APA: Copy

NOURI, R., AHANGARZADEH REZAEE, M., HASANI, A., SAGHAZADEH, M., ASGHARZADEH, M., & GHOJAZADEH, M.. (2016). EFFECT OF QRDR MUTATIONS ON CIPROFLOXACIN RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 16(2), 116-123. SID. https://sid.ir/paper/60097/en

Vancouver: Copy

NOURI R., AHANGARZADEH REZAEE M., HASANI A., SAGHAZADEH M., ASGHARZADEH M., GHOJAZADEH M.. EFFECT OF QRDR MUTATIONS ON CIPROFLOXACIN RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2016;16(2):116-123. Available from: https://sid.ir/paper/60097/en

IEEE: Copy

R. NOURI, M. AHANGARZADEH REZAEE, A. HASANI, M. SAGHAZADEH, M. ASGHARZADEH, and M. GHOJAZADEH, “EFFECT OF QRDR MUTATIONS ON CIPROFLOXACIN RESISTANCE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 16, no. 2, pp. 116–123, 2016, [Online]. Available: https://sid.ir/paper/60097/en

Related Journal Papers