CASPASE-MEDIATED APOPTOSIS IN SENSORY NEURONS OF CULTURED DORSAL ROOT GANGLIA IN ADULT MOUSE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOMENI HAMID REZA; SOLEIMANI MEHRANJANI MALEK; SHARIATZADEH MOHAMMAD ALI; HADDADI MAHNAZ

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2013 | Volume:15 | Issue:3 (59) Page(s): 212-217

Download Full-Text

View:

329

Download:

230

Cites:

Information Journal Paper

Title

CASPASE-MEDIATED APOPTOSIS IN SENSORY NEURONS OF CULTURED DORSAL ROOT GANGLIA IN ADULT MOUSE

Author(s)

MOMENI HAMID REZA | SOLEIMANI MEHRANJANI MALEK | SHARIATZADEH MOHAMMAD ALI | HADDADI MAHNAZ | Issue Writer Certificate

Keywords

APOPTOSIS

CASPASE

DORSAL ROOT GANGLIA

SENSORY NEURONS

Abstract

Objective: SENSORY NEURONS in DORSAL ROOT GANGLIA (DRG) undergo APOPTOSIS after peripheral nerve injury. The aim of this study was to investigate sensory neuron death and the mechanism involved in the death of these neurons in cultured DRG.Materials and Methods: In this experimental study, L5 DRG from adult mouse were dissected and incubated in culture medium for 24, 48, 72 and 96 hours. Freshly dissected and cultured DRG were then fixed and sectioned using a cryostat. Morphological and biochemical features of APOPTOSIS were investigated using fluorescent staining (Propidium iodide and Hoechst 33342) and the terminal Deoxynucleotide transferase dUTP nick end labeling (TUNEL) method respectively. To study the role of CASPASEs, general CASPASE inhibitor (Z-VAD. fmk, 100 mM) and immunohistochemistry for activated CASPASE-3 were used.Results: After 24, 48, 72 and 96 hours in culture, SENSORY NEURONS not only displayed morphological features of APOPTOSIS but also they appeared TUNEL positive. The application of Z-VAD. fmk inhibited APOPTOSIS in these neurons over the same time period. In addition, intense activated CASPASE-3 immunoreactivity was found both in the cytoplasm and the nuclei of these neurons after 24 and 48 hours.Conclusion: Results of the present study show CASPASE-dependent APOPTOSIS in the SENSORY NEURONS of cultured DRG from adult mouse.

Cites

No record.

References

Cite

APA: Copy

MOMENI, HAMID REZA, SOLEIMANI MEHRANJANI, MALEK, SHARIATZADEH, MOHAMMAD ALI, & HADDADI, MAHNAZ. (2013). CASPASE-MEDIATED APOPTOSIS IN SENSORY NEURONS OF CULTURED DORSAL ROOT GANGLIA IN ADULT MOUSE. CELL JOURNAL (YAKHTEH), 15(3 (59)), 212-217. SID. https://sid.ir/paper/610197/en

Vancouver: Copy

MOMENI HAMID REZA, SOLEIMANI MEHRANJANI MALEK, SHARIATZADEH MOHAMMAD ALI, HADDADI MAHNAZ. CASPASE-MEDIATED APOPTOSIS IN SENSORY NEURONS OF CULTURED DORSAL ROOT GANGLIA IN ADULT MOUSE. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(3 (59)):212-217. Available from: https://sid.ir/paper/610197/en

IEEE: Copy

HAMID REZA MOMENI, MALEK SOLEIMANI MEHRANJANI, MOHAMMAD ALI SHARIATZADEH, and MAHNAZ HADDADI, “CASPASE-MEDIATED APOPTOSIS IN SENSORY NEURONS OF CULTURED DORSAL ROOT GANGLIA IN ADULT MOUSE,” CELL JOURNAL (YAKHTEH), vol. 15, no. 3 (59), pp. 212–217, 2013, [Online]. Available: https://sid.ir/paper/610197/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops