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Information Journal Paper

Title

COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS

Pages

  218-223

Abstract

 Objective: It is believed that MONOCYTE isolation methods and maturation factors affect the phenotypic and functional characteristics of resultant DENDRITIC CELLS (DC). In the present study, we compared two MONOCYTE isolation methods, including plastic ADHERENCE-dendritic cells (Adh-DC) and magnetic activated cell sorting- DENDRITIC CELLS (MACS-DC), and their effects on phagocytic activity of differentiated immature DCs (imm DCs).Materials and Methods: In this experimental study, imm DCs were generated from plastic ADHERENCE and MACS isolated MONOCYTEs in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) in five days. The phagocytic activity of imm DCs was analyzed by fluorescein isothiocyanate (FITC)-conjugated latex bead using flow cytometry. One way ANOVA test was used for statistical analysis of differences among experimental groups, including Adh-DC and MACS-DC groups.Results: We found that phagocytic activity of Adh-DC was higher than MACS-DC, whereas the mean fluorescence intensity (MFI) of phagocytic cells was higher in MACS-DC (p<0.05).Conclusion: We concluded that it would be important to consider PHAGOCYTOSIS parameters of generated DCs before making any decision about MONOCYTE isolation methods to have fully functional DCs.

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    APA: Copy

    DELIREZH, NOWRUZ, SHOJAEEFAR, EHSAN, PARVIN, PARVA, & ASADI, BEHNAZ. (2013). COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS. CELL JOURNAL (YAKHTEH), 15(3 (59)), 218-223. SID. https://sid.ir/paper/610388/en

    Vancouver: Copy

    DELIREZH NOWRUZ, SHOJAEEFAR EHSAN, PARVIN PARVA, ASADI BEHNAZ. COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(3 (59)):218-223. Available from: https://sid.ir/paper/610388/en

    IEEE: Copy

    NOWRUZ DELIREZH, EHSAN SHOJAEEFAR, PARVA PARVIN, and BEHNAZ ASADI, “COMPARISON THE EFFECTS OF TWO MONOCYTE ISOLATION METHODS, PLASTIC ADHERENCE AND MAGNETIC ACTIVATED CELL SORTING METHODS, ON PHAGOCYTIC ACTIVITY OF GENERATED DENDRITIC CELLS,” CELL JOURNAL (YAKHTEH), vol. 15, no. 3 (59), pp. 218–223, 2013, [Online]. Available: https://sid.ir/paper/610388/en

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