FREQUENCY OF CLASS I AND II INTEGRONS IN METALOBETALACTAMASE PRODUCING CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

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KERAMATI N.; ZEIGHAMI H.; HAGHI F.

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Journal: Journal of Advances in Medical and Biomedical Research Year:2014 | Volume:22 | Issue:94 Page(s): 111-119

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Title

FREQUENCY OF CLASS I AND II INTEGRONS IN METALOBETALACTAMASE PRODUCING CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

Author(s)

KERAMATI N. | ZEIGHAMI H. | HAGHI F. | Issue Writer Certificate

Keywords

INTEGRONQ2

MTALLO-BETA-LACTAMASEQ2

PSEUDOMONAS AERUGINOSAQ2

Abstract

Background and Objective: PSEUDOMONAS AERUGINOSAis an opportunistic nosocomial pathogen. Evidence suggests that the incidence of enzyme-producing strains ofPseudomonas aeruginosa Metalo Beta Lactamases (MBL) is a major problem in the treatment of infections caused by this organism. The aim of this study was to investigate the frequency of class I and II INTEGRONs among metalobetalactamase producing PSEUDOMONAS AERUGINOSAisolated from clinical samples from Zanjan hospitals.Materials and Methods: In this cross-sectional study, 120 PSEUDOMONAS AERUGINOSA isolates from clinical specimens were collected from Zanjan hospitals in 2012-2013. After verifying isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Baur method) was performed according to CLSI guidelines against 7 antibiotics. The DDST was then carried out for detection of MBLs. Finally, the presence ofIntI1, IntI2, blaVIM and bla GIM genes was determined by PCR.Results: In our study, the highest and the lowest resistant patterns were related to Cefotaxime and Amikacin with 43.3% and 21.6%, respectively. Of 120 isolates, the metallo- beta-lactamase was detected in 35 (29%) isolates. According to results, the frequency ofintI1, intI2, bla VIM genes in MBL producers were 32 (91%), 3 (9%), and 6 (17%), respectively, but nobla GIM positive isolate was detected. Also all of IntI2 positive isolates carriedIntI1 and bla VIM, simultaneously.Conclusion: The results of this study indicate high frequency of class I INTEGRON gene among MBL producingPseudomonas aeruginosa isolates. Due to the importance of INTEGRONs in dissemination of antibiotic resistance genes, detection and prevention of these elements are necessary.

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APA: Copy

KERAMATI, N., ZEIGHAMI, H., & HAGHI, F.. (2014). FREQUENCY OF CLASS I AND II INTEGRONS IN METALOBETALACTAMASE PRODUCING CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 22(94), 111-119. SID. https://sid.ir/paper/61666/en

Vancouver: Copy

KERAMATI N., ZEIGHAMI H., HAGHI F.. FREQUENCY OF CLASS I AND II INTEGRONS IN METALOBETALACTAMASE PRODUCING CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2014;22(94):111-119. Available from: https://sid.ir/paper/61666/en

IEEE: Copy

N. KERAMATI, H. ZEIGHAMI, and F. HAGHI, “FREQUENCY OF CLASS I AND II INTEGRONS IN METALOBETALACTAMASE PRODUCING CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 22, no. 94, pp. 111–119, 2014, [Online]. Available: https://sid.ir/paper/61666/en

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