POLYMERASE CHAIN REACTION PURIFICATION

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SHAHHOSSEINI FATIMA

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ISSN: 2588-4824

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PHYSIOLOGY Year:2011 | Volume:1 | Issue:3 Page(s): 205-206

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Title

POLYMERASE CHAIN REACTION PURIFICATION

Author(s)

SHAHHOSSEINI FATIMA | Issue Writer Certificate

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Abstract

What is PCR purification.PCR Purification Protocol is designed to purify double stranded DNA fragments from PCR and other enzymatic reactions. Fragments from 100bp to 10kb are purified from primers, nucleotides, polymerases and salts using QIAquick spin columns in a microcentrifuge. Even if distinct bands of the expected size are observed, primer dimers should be removed by PCR purification method. In the absence of the purification, the proofreading activity of DNA polymerase will degrade the PCR fragments or remove the 3' terminal deoxyadenosine from the vector during ligation reaction.

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APA: Copy

SHAHHOSSEINI, FATIMA. (2011). POLYMERASE CHAIN REACTION PURIFICATION. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 1(3), 205-206. SID. https://sid.ir/paper/624830/en

Vancouver: Copy

SHAHHOSSEINI FATIMA. POLYMERASE CHAIN REACTION PURIFICATION. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2011;1(3):205-206. Available from: https://sid.ir/paper/624830/en

IEEE: Copy

FATIMA SHAHHOSSEINI, “POLYMERASE CHAIN REACTION PURIFICATION,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 1, no. 3, pp. 205–206, 2011, [Online]. Available: https://sid.ir/paper/624830/en

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