MICROPROPAGATION OF ASTRAGALUS ADSCENDENS: A SOURCE OF GAZ-ANGABIN MANNA IN IRAN (PERSIAN MANNA)

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ESMAEILI GH.; AZIZI M.; AROEI H.; SAMIEI L.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2016 | Volume:18 | Issue:3 Page(s): 741-750

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Information Journal Paper

Title

MICROPROPAGATION OF ASTRAGALUS ADSCENDENS: A SOURCE OF GAZ-ANGABIN MANNA IN IRAN (PERSIAN MANNA)

Author(s)

ESMAEILI GH. | AZIZI M. | AROEI H. | SAMIEI L. | Issue Writer Certificate

Keywords

APICAL BUDSQ3

CALLUS INDUCTIONQ3

CLONAL PROPAGATIONQ3

GAVAN-E-GAZ-ANGABINQ3

PROLIFERATIONQ3

Abstract

The aim of this study was to investigate thein vitro PROLIFERATION of Astragalus adscendens. Explants were taken from hypocotyl and cotyledon and were cultured on the basic medium of Murashige and Skoog (MS) complemented with various plant growth regulators, (NAA, BAP, KIN, ZEA), to induce direct shoot regeneration. CALLUS INDUCTION was significantly affected by different concentrations of PGRs. Callus formation was observed from hypocotyl explants, but they were not induced to adventitious shoot regeneration and most of them were turned into brown. Therefore, rapid multiplication, performed using shoot APICAL BUDS, and obtained from 15-day old sterile seedlings. APICAL BUDS were cultured on MS medium containing various levels of BAP, KIN and ZEA (0.5, 1.0, 2.0 and 4.0 mg L-1) alone or in combination with 0.5 mg L-1 NAA. The highest number of shoot regenerants (8.5/explants) and leaves (22.4/explants) obtained on MS medium with 4 mg L-1 BAP. The highest root induction (100%) was obtained from MS media supplemented with 0.3 mg L-1 NAA. Rooted plantlets were successfully acclimatized in pots with 1: 1: 1 mixture of soil, peat, and perlite.

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APA: Copy

ESMAEILI, GH., AZIZI, M., AROEI, H., & SAMIEI, L.. (2016). MICROPROPAGATION OF ASTRAGALUS ADSCENDENS: A SOURCE OF GAZ-ANGABIN MANNA IN IRAN (PERSIAN MANNA). JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 18(3), 741-750. SID. https://sid.ir/paper/62531/en

Vancouver: Copy

ESMAEILI GH., AZIZI M., AROEI H., SAMIEI L.. MICROPROPAGATION OF ASTRAGALUS ADSCENDENS: A SOURCE OF GAZ-ANGABIN MANNA IN IRAN (PERSIAN MANNA). JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2016;18(3):741-750. Available from: https://sid.ir/paper/62531/en

IEEE: Copy

GH. ESMAEILI, M. AZIZI, H. AROEI, and L. SAMIEI, “MICROPROPAGATION OF ASTRAGALUS ADSCENDENS: A SOURCE OF GAZ-ANGABIN MANNA IN IRAN (PERSIAN MANNA),” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 18, no. 3, pp. 741–750, 2016, [Online]. Available: https://sid.ir/paper/62531/en

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