CONSTRUCTION OF A PROBIOTIC LACTIC ACID BACTERIUM THAT EXPRESSES ACID-RESISTANT PHYTASE ENZYME

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MAJIDZADEH HERAVI R.; SANKIAN M.; KERMANSHAHI H.; NASSIRI M.R.; HERAVI MOUSSAVI A.; ROOZBEH NASIRAII L.; VARASTEH A.R.

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Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2016 | Volume:18 | Issue:4 Page(s): 925-936

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Title

CONSTRUCTION OF A PROBIOTIC LACTIC ACID BACTERIUM THAT EXPRESSES ACID-RESISTANT PHYTASE ENZYME

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Keywords

LACTOBACILLUS SALIVARUSQ3

LACTOCOCCUS LACTISQ3

RECOMBINANTQ3

PHYTASEQ3

POULTRYQ3

Abstract

The use of genetically engineered probiotics to express specific enzymes has been the subject of considerable attention in POULTRY industry because of increased nutrient availability and reduced cost of enzyme supplementation. PHYTASE enzyme is commonly added to POULTRY feed to improve digestibility and availability of phosphorus from plant sources. To construct a probiotic with potential of phytate degradation, PHYTASE gene (appA) from E. coli was cloned and transformed into two probiotic bacteria Lactobacillus salivariusand LACTOCOCCUS LACTIS. The results showed plasmid instability, unable to express the gene. The expression of app A gene in L. lactis was analyzed by detecting specific RNA and zymography assay. PHYTASE enzyme was isolated from cellular extracts of RECOMBINANTL. lactis, showing a 46 kDa band upon the SDS-PAGE analysis. Zymogram also confirmed the PHYTASE activity of the 46 kDa band corresponding to the enzyme. An enzyme activity of 4.9 U mL-1 was obtained in cell extracts of L. lactis. The growth of native and RECOMBINANTL. lactis was similar in the presence of two concentrations of ox bile.

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APA: Copy

MAJIDZADEH HERAVI, R., SANKIAN, M., KERMANSHAHI, H., NASSIRI, M.R., HERAVI MOUSSAVI, A., ROOZBEH NASIRAII, L., & VARASTEH, A.R.. (2016). CONSTRUCTION OF A PROBIOTIC LACTIC ACID BACTERIUM THAT EXPRESSES ACID-RESISTANT PHYTASE ENZYME. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 18(4), 925-936. SID. https://sid.ir/paper/62604/en

Vancouver: Copy

MAJIDZADEH HERAVI R., SANKIAN M., KERMANSHAHI H., NASSIRI M.R., HERAVI MOUSSAVI A., ROOZBEH NASIRAII L., VARASTEH A.R.. CONSTRUCTION OF A PROBIOTIC LACTIC ACID BACTERIUM THAT EXPRESSES ACID-RESISTANT PHYTASE ENZYME. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2016;18(4):925-936. Available from: https://sid.ir/paper/62604/en

IEEE: Copy

R. MAJIDZADEH HERAVI, M. SANKIAN, H. KERMANSHAHI, M.R. NASSIRI, A. HERAVI MOUSSAVI, L. ROOZBEH NASIRAII, and A.R. VARASTEH, “CONSTRUCTION OF A PROBIOTIC LACTIC ACID BACTERIUM THAT EXPRESSES ACID-RESISTANT PHYTASE ENZYME,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 18, no. 4, pp. 925–936, 2016, [Online]. Available: https://sid.ir/paper/62604/en

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