IN-HOUSE PRODUCTION OF LACTOSE-HYDROLYSED MILK BY BETA-GALACTOSIDASE FROM LACTOBACILLUS BULGARICUS

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JOKAR A.; KARBASSI A.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2011 | Volume:13 | Issue:4 Page(s): 577-584

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Title

IN-HOUSE PRODUCTION OF LACTOSE-HYDROLYSED MILK BY BETA-GALACTOSIDASE FROM LACTOBACILLUS BULGARICUS

Author(s)

JOKAR A. | KARBASSI A. | Issue Writer Certificate

Keywords

BETA-GALACTOSIDASEQ3

CRUDE ENZYMATIC EXTRACTQ3

LACTOBACILLUS BULGARICUSQ3

LACTOSE HYDROLYSISQ3

Abstract

Crude Enzyme (BETA-GALACTOSIDASE) Extract (CEE) was produced by Lactobacillus ssp. bulgaricus CHR Hansen Lb-12 and was applied in sterile milk which had been processed through Ultra High Temperature method (UHT milk), for hydrolyzing lactose. Lactosehydrolyzed milk was also produced by a pure and commercially available betagalactosidase (Maxilact). Optimum quantities of CEE and Maxilact enzyme, for producing lactose-hydrolyzed milk, during 6 hours of processing, were 0.418 and 0.512 U ml-1, respectively. Using more than 0.418 U ml-1 CEE resulted in unacceptable acidity. Acidity of lactose-hydrolyzed milk produced through 0.418 U ml-1 of CEE was significantly increased from 15 to 17oD, while enhancement of acidity in lactosehydrolyzed milk produced through Maxilact enzyme was not significant. Total count of lactose-hydrolyzed milk by 0.418 U ml-1 CEE, after 6 hours of processing was significantly increased from 5 to 30 CFU (Colony Forming Unit). Sensory evaluation of lactosehydrolyzed milk and ordinary UHT milk (as control) did not show any significant differences with respect to acceptability of sweetness, taste, aftertaste and color.

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APA: Copy

JOKAR, A., & KARBASSI, A.. (2011). IN-HOUSE PRODUCTION OF LACTOSE-HYDROLYSED MILK BY BETA-GALACTOSIDASE FROM LACTOBACILLUS BULGARICUS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 13(4), 577-584. SID. https://sid.ir/paper/62666/en

Vancouver: Copy

JOKAR A., KARBASSI A.. IN-HOUSE PRODUCTION OF LACTOSE-HYDROLYSED MILK BY BETA-GALACTOSIDASE FROM LACTOBACILLUS BULGARICUS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2011;13(4):577-584. Available from: https://sid.ir/paper/62666/en

IEEE: Copy

A. JOKAR, and A. KARBASSI, “IN-HOUSE PRODUCTION OF LACTOSE-HYDROLYSED MILK BY BETA-GALACTOSIDASE FROM LACTOBACILLUS BULGARICUS,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 13, no. 4, pp. 577–584, 2011, [Online]. Available: https://sid.ir/paper/62666/en

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