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Title

OVER - EXPRESSION EFFECT OF GENE ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ON PRODUCTION OF TAXOL IN IRANIAN HAZEL (CORYLUS AVELLANA L.)

Pages

  100-110

Abstract

 Background: Sustainable and commercial production of taxol as an anti-cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol.Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production.Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis.Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively.Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.

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    APA: Copy

    QADERI, A., OMIDI, M., ZEBARJADI, A.R., & HAJIAGHAEE, R.. (2013). OVER - EXPRESSION EFFECT OF GENE ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ON PRODUCTION OF TAXOL IN IRANIAN HAZEL (CORYLUS AVELLANA L.). JOURNAL OF MEDICINAL PLANTS, 12(47), 100-110. SID. https://sid.ir/paper/628584/en

    Vancouver: Copy

    QADERI A., OMIDI M., ZEBARJADI A.R., HAJIAGHAEE R.. OVER - EXPRESSION EFFECT OF GENE ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ON PRODUCTION OF TAXOL IN IRANIAN HAZEL (CORYLUS AVELLANA L.). JOURNAL OF MEDICINAL PLANTS[Internet]. 2013;12(47):100-110. Available from: https://sid.ir/paper/628584/en

    IEEE: Copy

    A. QADERI, M. OMIDI, A.R. ZEBARJADI, and R. HAJIAGHAEE, “OVER - EXPRESSION EFFECT OF GENE ENCODING 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE ON PRODUCTION OF TAXOL IN IRANIAN HAZEL (CORYLUS AVELLANA L.),” JOURNAL OF MEDICINAL PLANTS, vol. 12, no. 47, pp. 100–110, 2013, [Online]. Available: https://sid.ir/paper/628584/en

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