A CONSISTENT PCR-RFLP ASSAY BASED ON ITS-2 RIBOSOMAL DNA FOR DIFFERENTIATION OF FASCIOLA SPECIES

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SHAFIEI REZA; SARKARI BAHADOR; MOSHFE ABDOLALI

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2013 | Volume:16 | Issue:12 Page(s): 1266-1269

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Title

A CONSISTENT PCR-RFLP ASSAY BASED ON ITS-2 RIBOSOMAL DNA FOR DIFFERENTIATION OF FASCIOLA SPECIES

Author(s)

SHAFIEI REZA | SARKARI BAHADOR | MOSHFE ABDOLALI | Issue Writer Certificate

Keywords

DIFFERENTIATION

FASCIOLA

RESTRICTION ENZYMES

RFLP

Abstract

Objective (s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of FASCIOLA hepatica and FASCIOLA gigantica. DIFFERENTIATION of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on ITS2 of the ribosomal DNA genes from these two species.Materials and Methods: Adult FASCIOLA spp. were isolated from bile duct of naturally infected animals. The species of FASCIOLA were confirmed by sequencing the 505 bp region of the ITS2 gene in the isolates. By running the sequences of the samples in NEBcutter, suitable RESTRICTION ENZYMES (MspI and KpnI) were selected. Eight F. gigantica and eighteen F. hepatica samples were evaluated.Results: While RFLP pattern with MspI produced a profile by which it was difficult to differentiate these two species, KpnI along with MspI, produced a consistent pattern of a 231, 212 and 93 bp fragments in F. hepatica. This pattern was not seen in F. gigantica.Conclusion: Findings of this study demonstrated that RFLP with KpnI and MspI produce a suitable pattern which simply differentiates F. hepatica from F. gigantica.

Cites

EPIDEMIOLOGY OF HUMAN FASCIOLIASIS AND INTESTINAL HELMINTHES IN RURAL AREAS OF BOYER-AHMAD TOWNSHIP, SOUTHWEST IRAN; A POPULATION BASED STUDY

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APA: Copy

SHAFIEI, REZA, SARKARI, BAHADOR, & MOSHFE, ABDOLALI. (2013). A CONSISTENT PCR-RFLP ASSAY BASED ON ITS-2 RIBOSOMAL DNA FOR DIFFERENTIATION OF FASCIOLA SPECIES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 16(12), 1266-1269. SID. https://sid.ir/paper/630938/en

Vancouver: Copy

SHAFIEI REZA, SARKARI BAHADOR, MOSHFE ABDOLALI. A CONSISTENT PCR-RFLP ASSAY BASED ON ITS-2 RIBOSOMAL DNA FOR DIFFERENTIATION OF FASCIOLA SPECIES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2013;16(12):1266-1269. Available from: https://sid.ir/paper/630938/en

IEEE: Copy

REZA SHAFIEI, BAHADOR SARKARI, and ABDOLALI MOSHFE, “A CONSISTENT PCR-RFLP ASSAY BASED ON ITS-2 RIBOSOMAL DNA FOR DIFFERENTIATION OF FASCIOLA SPECIES,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 16, no. 12, pp. 1266–1269, 2013, [Online]. Available: https://sid.ir/paper/630938/en

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