SHORT COMMUNICATION: GENETIC POLYMORPHISM OF GDF9 GENE IN IRANIAN MOGHANI SHEEP BREED

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ALA NOSHAHR F.; RAFAT A.

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Paper Information

Journal: IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE Year:2014 | Volume:4 | Issue:4 Page(s): 887-890

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Title

SHORT COMMUNICATION: GENETIC POLYMORPHISM OF GDF9 GENE IN IRANIAN MOGHANI SHEEP BREED

Author(s)

ALA NOSHAHR F. | RAFAT A. | Issue Writer Certificate

Keywords

GDF9

MOGHANI SHEEP

PCR

POLYMORPHISM

Abstract

The families of TGF-b proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. Three related oocyte-derived members of the transforming growth factor- b superfamily namely growth differentiation factor 9 (GDF9), BMP15 and BMPR-IB have been shown to be essential for follicular growth and ovulation. Different mutations in the GDF9 gene cause increased ovulation rate and infertility in a dosage-sensitive manner in sheep. In this study, blood samples were collected from 150 MOGHANI SHEEP breed using venojects treated with the anti-clot substance (EDTA) and subsequently their DNA content were salted out and extracted. The quantity and quality of extracted DNA was examined using spectrophotometery and gel electrophoresis, respectively. With using a pair of specific primers, the DNA fragment was amplified from exon 1 of GDF9 (462 bp). Digested PCR products with HhaI enzyme showed a G to A substiuation in GDF9 locus. The wild type allele of this gene (G/+) with two restriction site resulted DNA fragments of 156, 52 and 254 bp while the mutant allele (G/-) with one restriction site resulted two DNA fragments with the size of 52 and 410 bp. Genotype frequencies for G (+/+), G (+/-) and G (-/-) were 0.66, 0.24 and 0.1, respectively. From studied luci, GDF9 was polymorphic in Iranian MOGHANI SHEEP breed.

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APA: Copy

ALA NOSHAHR, F., & RAFAT, A.. (2014). SHORT COMMUNICATION: GENETIC POLYMORPHISM OF GDF9 GENE IN IRANIAN MOGHANI SHEEP BREED. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(4), 887-890. SID. https://sid.ir/paper/636317/en

Vancouver: Copy

ALA NOSHAHR F., RAFAT A.. SHORT COMMUNICATION: GENETIC POLYMORPHISM OF GDF9 GENE IN IRANIAN MOGHANI SHEEP BREED. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2014;4(4):887-890. Available from: https://sid.ir/paper/636317/en

IEEE: Copy

F. ALA NOSHAHR, and A. RAFAT, “SHORT COMMUNICATION: GENETIC POLYMORPHISM OF GDF9 GENE IN IRANIAN MOGHANI SHEEP BREED,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 4, no. 4, pp. 887–890, 2014, [Online]. Available: https://sid.ir/paper/636317/en

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