MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT

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ELMI AKHONI S.

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Journal: Studies in Medical Sciences Year:2000 | Volume:11 | Issue:1 Page(s): 35-41

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Title

MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT

Author(s)

ELMI AKHONI S. | Issue Writer Certificate

Keywords

ENZYMEQ3

ACETYLCHOLINESTERASEQ4

SUCROSE GRADIENTQ4

Abstract

Molecular weight determination of proteins especially ENZYMEs are the most routine and popular method in Enzmology. For molecular weight and its subunits determination, a homogenous wistar Rat Brain was prepared in a phosphate buffer. After centrifugation, supernatant which contained, "soluble ENZYME" was isolated. The second stage, pellet was brought to the original volume with phosphate buffer contained Triton X-100 with a certain concentration.After second Centrifugation in a certain RPM, the supernatant which is called "detergent soluble ENZYME" was isolated. At the same time SUCROSE GRADIENT in the plastic tubes were prepared and applied on to the top Gradient and centrifused for over night. The next day all plastic tubes were put on the stand and samples were prepared through a small hole made in the buttom of this plastic tubes and finally the ENZYME activity were determined.

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APA: Copy

ELMI AKHONI, S.. (2000). MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), 11(1), 35-41. SID. https://sid.ir/paper/63805/en

Vancouver: Copy

ELMI AKHONI S.. MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT. STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES)[Internet]. 2000;11(1):35-41. Available from: https://sid.ir/paper/63805/en

IEEE: Copy

S. ELMI AKHONI, “MOLECULAR WEIGHT DTERMLNATION AND ACETYLCHOLINESTERASE USING SUCROSE GRADIENT,” STUDIES IN MEDICAL SCIENCE (THE JOURNAL OF URMIA UNIVERSITY OF MEDICAL SCINCES), vol. 11, no. 1, pp. 35–41, 2000, [Online]. Available: https://sid.ir/paper/63805/en

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