MOLECULAR IDENTIFICATION OF FASCIOLA SPECIES IN WEST AZERBAIJAN PROVINCE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GALAVANI HOSSEIN; GHOLIZADEH SABER; HAZRATI TAPPEH KHOSROW

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Studies in Medical Sciences Year:2014 | Volume:25 | Issue:11 Page(s): 1033-1040

Download Full-Text

Persian Verion

View:

813

Download:

Cites:

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF FASCIOLA SPECIES IN WEST AZERBAIJAN PROVINCE

Author(s)

GALAVANI HOSSEIN | GHOLIZADEH SABER | HAZRATI TAPPEH KHOSROW | Issue Writer Certificate

Keywords

FASCIOLAQ2

ITS1Q2

ITS2Q3

5.8SQ3

PCRQ2

RIBOSOMAL DNAQ2

Abstract

Background & Aims: Fascioliasis caused by FASCIOLA hepatica and FASCIOLA gigantica has medical and economic importance in the world. Traditional approaches are not accurate and reliable in identification of agent parasites. Thus the present study was designed to identify the FASCIOLA sppby molecular methods in West Azerbaijan province.Materials & Methods: In current study FASCIOLA isolates were collected from slaughterhouses in five districts in West Azerbaijan province, Northwestern Iran. Parasite species were identified using morphological and molecular tools, RIBOSOMAL DNA ITS1, 5.8S and ITS2 sequences. A number of 580 adult FASCIOLA worms were isolated from 90 infected livers (50 liver of cattle, 40 liver of sheep). Out of 110 DNA extracted specimens, 50 specimens were subject to direct sequencing.Results: Sequence analysis showed 100% similarity in ITS1 (428 bp), 5.8S (158 bp) and ITS2 (366 bp) regions of all sequences. The degree of identity between F. hepatica and F. gigantica sequences was 98% with 11 nucleotide mismatches. Based on rDNA-ITS1 and ITS2 sequences, only F. hepatica flukes are scattered among cattle and sheep population in West Azerbaijan province. Finally, 150 sequence of F. hepatica (50 sequences of each region of ITS) from West Azerbaijan province were recorded to GenBank.Conclusion: The results of this study showed no evidence of F. giganticain cattle and sheep in West Azerbaijan province. More studies are essential to design new molecular markers will be helpful in correct species identification and therefore, for control and prevention of this parasitic disease.

Cites

No record.

References

No record.

Cite

APA: Copy

GALAVANI, HOSSEIN, GHOLIZADEH, SABER, & HAZRATI TAPPEH, KHOSROW. (1393). شناسایی مولکولی گونه های فاسیولا در استان آذربایجان غربی. مجله مطالعات علوم پزشکی (مجله پزشکی دانشگاه علوم پزشکی ارومیه), 25(11), 1033-1040. SID. https://sid.ir/paper/64260/en

Vancouver: Copy

GALAVANI HOSSEIN, GHOLIZADEH SABER, HAZRATI TAPPEH KHOSROW. شناسایی مولکولی گونه های فاسیولا در استان آذربایجان غربی. مجله مطالعات علوم پزشکی (مجله پزشکی دانشگاه علوم پزشکی ارومیه)[Internet]. 1393;25(11):1033-1040. Available from: https://sid.ir/paper/64260/en

IEEE: Copy

HOSSEIN GALAVANI, SABER GHOLIZADEH, and KHOSROW HAZRATI TAPPEH, “شناسایی مولکولی گونه های فاسیولا در استان آذربایجان غربی,” مجله مطالعات علوم پزشکی (مجله پزشکی دانشگاه علوم پزشکی ارومیه), vol. 25, no. 11, pp. 1033–1040, 1393, [Online]. Available: https://sid.ir/paper/64260/en

Related Journal Papers