EFFECT OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES-LABELING ON MOUSE EMBRYONIC STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

PARSA HAMED; SHAMSASENJAN KARIM; MOVASSAGHPOUR ALIAKBAR; AKBARZADEH PARVIN; AMOGHLI TABRIZI BAHRAM; DEHDILANI NIMA; LOTFINEGAD PARISA; SOLEIMANLOO FARZANEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:2 Page(s): 221-230

Download Full-Text

View:

409

Download:

355

Cites:

Information Journal Paper

Title

EFFECT OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES-LABELING ON MOUSE EMBRYONIC STEM CELLS

Author(s)

Keywords

IRON OXIDE

MOUSE EMBRYONIC STEM CELLS

CELL TRACKING

Abstract

Objective: Superparamagnetic IRON OXIDE nanoparticles (SPIONs) have been used to label mammalian cells and to monitor their fate in vivo using magnetic resonance imaging (MRI). However, the effectiveness of phenotype of labeled cells by SPIONs is still a matter of question. The aim of this study was to investigate the efficiency and biological effects of labeled MOUSE EMBRYONIC STEM CELLS (mESCs) using ferumoxide- protamine sulfate complex.Materials and Methods: In an experimental study, undifferentiated mESCs, C571 line, a generous gift of Stem Cell Technology Company, were cultured on gelatin-coated flasks. The proliferation and viability of SPION-labeled cells were compared with control. ESCs and embryoid bodies (EBs) derived from differentiated hematopoietic stem cells (HSCs) were analyzed for stage-specific cell surface markers using fluorescence-activated cell sorting (FACS).Results: Our observations showed that SPIONs have no effect on the self-renewal ability of mESCs. Reverse microscopic observations and prussian blue staining revealed 100% of cells were labeled with iron particles. SPION-labeled mESCs did not significantly alter cell viability and proliferation activity. Furthermore, labeling did not alter expression of representative surface phenotypic markers such as stage-specific embryonic antigen 1 (SSEA1) and cluster of differentiation 117 (CD117) on undifferentiated ESC and CD34, CD38 on HSCs, as measured by flowcytometry.Conclusion: According to the results of the present study, SPIONs-labeling method as MRI agents in mESCs has no negative effects on growth, morphology, viability, proliferation and differentiation that can be monitored in vivo, noninvasively. Non-invasive CELL TRACKING methods are considered as new perspectives in cell therapy for clinical use and as an easy method for evaluating the placement of stem cells after transplantation.

Cites

No record.

References

Cite

APA: Copy

PARSA, HAMED, SHAMSASENJAN, KARIM, MOVASSAGHPOUR, ALIAKBAR, AKBARZADEH, PARVIN, AMOGHLI TABRIZI, BAHRAM, DEHDILANI, NIMA, LOTFINEGAD, PARISA, & SOLEIMANLOO, FARZANEH. (2015). EFFECT OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES-LABELING ON MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH), 17(2), 221-230. SID. https://sid.ir/paper/644120/en

Vancouver: Copy

PARSA HAMED, SHAMSASENJAN KARIM, MOVASSAGHPOUR ALIAKBAR, AKBARZADEH PARVIN, AMOGHLI TABRIZI BAHRAM, DEHDILANI NIMA, LOTFINEGAD PARISA, SOLEIMANLOO FARZANEH. EFFECT OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES-LABELING ON MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(2):221-230. Available from: https://sid.ir/paper/644120/en

IEEE: Copy

HAMED PARSA, KARIM SHAMSASENJAN, ALIAKBAR MOVASSAGHPOUR, PARVIN AKBARZADEH, BAHRAM AMOGHLI TABRIZI, NIMA DEHDILANI, PARISA LOTFINEGAD, and FARZANEH SOLEIMANLOO, “EFFECT OF SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES-LABELING ON MOUSE EMBRYONIC STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 17, no. 2, pp. 221–230, 2015, [Online]. Available: https://sid.ir/paper/644120/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops