DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS BY USING A LENTIVIRAL VECTOR CARRYING PDX1

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ALLAHVERDI AMIR; ABROUN SAIED; JAFARIAN AREFEH; SOLEIMANI MASOUD; TAGHIKHANI MOHAMMAD; ESKANDARI FATEMEH

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Journal: Cell Journal (Yakhteh) Year:2015 | Volume:17 | Issue:2 Page(s): 231-242

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Title

DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS BY USING A LENTIVIRAL VECTOR CARRYING PDX1

Author(s)

Keywords

PDX1

DIABETES TYPE I

MENESCHYMAL STEM CELLS

Abstract

Type I diabetes is an immunologically-mediated devastation of insulin producing cells (IPCs) in the pancreatic islet. Stem cells that produce b-cells are a new promising tool. Adult stem cells such as mesenchymal stem cells (MSCs) are self renewing multi potent cells showing capabilities to differentiate into ectodermal, mesodermal and endodermal tissues. Pancreatic and duodenal homeobox factor 1 (PDX1) is a master regulator gene required for embryonic development of the pancreas and is crucial for normal pancreatic islets activities in adults.Materials and Methods: We induced the over-expression of the PDX1 gene in human bone marrow MSCs (BM-MSCs) by Lenti-PDX1 in order to generate IPCs. Next, we examine the ability of the cells by measuring insulin/c-peptide production and INSULIN and PDX1 gene expressions.Results: After transduction, MSCs changed their morphology at day 5 and gradually differentiated into IPCs. INSULIN and PDX1 expressions were confirmed by real time polymerase chain reaction (RT-PCR) and immunostaining. IPC secreted insulin and C-peptide in the media that contained different glucose concentrations.Conclusion: MSCs differentiated into IPCs by genetic manipulation. Our result showed that lentiviral vectors could deliver PDX1 gene to MSCs and induce pancreatic differentiation.

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APA: Copy

ALLAHVERDI, AMIR, ABROUN, SAIED, JAFARIAN, AREFEH, SOLEIMANI, MASOUD, TAGHIKHANI, MOHAMMAD, & ESKANDARI, FATEMEH. (2015). DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS BY USING A LENTIVIRAL VECTOR CARRYING PDX1. CELL JOURNAL (YAKHTEH), 17(2), 231-242. SID. https://sid.ir/paper/648529/en

Vancouver: Copy

ALLAHVERDI AMIR, ABROUN SAIED, JAFARIAN AREFEH, SOLEIMANI MASOUD, TAGHIKHANI MOHAMMAD, ESKANDARI FATEMEH. DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS BY USING A LENTIVIRAL VECTOR CARRYING PDX1. CELL JOURNAL (YAKHTEH)[Internet]. 2015;17(2):231-242. Available from: https://sid.ir/paper/648529/en

IEEE: Copy

AMIR ALLAHVERDI, SAIED ABROUN, AREFEH JAFARIAN, MASOUD SOLEIMANI, MOHAMMAD TAGHIKHANI, and FATEMEH ESKANDARI, “DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS BY USING A LENTIVIRAL VECTOR CARRYING PDX1,” CELL JOURNAL (YAKHTEH), vol. 17, no. 2, pp. 231–242, 2015, [Online]. Available: https://sid.ir/paper/648529/en

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