IMPROVING THE YIELD OPERATION BASED ON THE EFFECT OF HYDRODYNAMICS IN GLUTAMIC ACID FERMENTATION

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TABATABAEI M.; ROUSTAAZAD R.; VOSOUGHI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: AMIRKABIR Year:2003 | Volume:14 | Issue:53 Page(s): 323-329

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Title

IMPROVING THE YIELD OPERATION BASED ON THE EFFECT OF HYDRODYNAMICS IN GLUTAMIC ACID FERMENTATION

Author(s)

TABATABAEI M. | ROUSTAAZAD R. | VOSOUGHI M. | Issue Writer Certificate

Keywords

GLUTAMIC ACIDQ3

FERMEATATIONQ4

CORYNEBACTERIUM GLUTAMICANQ3

AIRLIFT-BEFFLED BIOREACTORQ4

Abstract

Production of GLUTAMIC ACID via fermentation of C. glutamicum was studied. Among the parameters which affect the bacterial growth and GA production, are the medium composition, dissolved oxygen and hydrodynamics. Therefore, effect of change in the broth constituents, rate of aeration and type of the bioreactor including shake flask, stirred bioreactors and airlift were investigated. At the first stage, in the shake flask, sugar consumption and biomass production was checked in the media based on beet and cane molasses. In the next step, a fed batch protocol in which a 12% ammonia solution was used to set the pH was studied in the stirred bioreactor. Following this, due to the too low sugar concentration at the onset of the production phase, double and mixed fed batch modes of operation in which aside from ammonia, molasses was fed to the fermenter at the specific rate was tested. In the stirred bioreactor, the best result in the mixed fed batch and at the optimum conditions namely aeration rate of 1.2 vvm and rotation speed of 500pm was as high as 10g/l of GLUTAMIC ACID. At the 3rd stage, result of the stirred bioreactor was compared against that of an Airlift fermenter. In the ALF. in the aeration rates from 1.2-3vvm, the best production was achieved in the maximum aeration as 18/l and the best production vs. energy Consumption was at the 2vvm aeration as 1989/energy. At the 2vvm aeration, production in the beet and cane molasses based media were compared in the ALF. The best result was obtained in the medoum based on cane molasses which was as high as 30g/l.

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APA: Copy

TABATABAEI, M., ROUSTAAZAD, R., & VOSOUGHI, M.. (2003). IMPROVING THE YIELD OPERATION BASED ON THE EFFECT OF HYDRODYNAMICS IN GLUTAMIC ACID FERMENTATION . AMIRKABIR, 14(53), 323-329. SID. https://sid.ir/paper/670/en

Vancouver: Copy

TABATABAEI M., ROUSTAAZAD R., VOSOUGHI M.. IMPROVING THE YIELD OPERATION BASED ON THE EFFECT OF HYDRODYNAMICS IN GLUTAMIC ACID FERMENTATION . AMIRKABIR[Internet]. 2003;14(53):323-329. Available from: https://sid.ir/paper/670/en

IEEE: Copy

M. TABATABAEI, R. ROUSTAAZAD, and M. VOSOUGHI, “IMPROVING THE YIELD OPERATION BASED ON THE EFFECT OF HYDRODYNAMICS IN GLUTAMIC ACID FERMENTATION ,” AMIRKABIR, vol. 14, no. 53, pp. 323–329, 2003, [Online]. Available: https://sid.ir/paper/670/en

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