IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL

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FAZILI AFSANEH; GHOLAMI SOGHRA; MINAIE ZANGI BAGHER; SEYEDJAFARI EHSAN; GHOLAMI MAHDI

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Journal: Cell Journal (Yakhteh) Year:2016 | Volume:18 | Issue:3 (71) Page(s): 310-321

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Title

IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL

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Keywords

DIFFERENTIATION

TRANSPLANTATION

SCAFFOLD

INSULIN

Abstract

Objective: This study examined the in vivo DIFFERENTIATION of mesenchymal stem cells (MSCs) into INSULIN producing cells (IPCs) on electrospun poly-L-lactide acid (PLLA) SCAFFOLDs coated withMatricaria chammomila L. (chamomile) oil.Materials and Methods: In this interventional, experimental study adipose MSCs (AMSCs) were isolated from 12 adult male New Zealand white rabbits and characterized by flow cytometry. AMSCs were subsequently differentiated into osteogenic and adipogenic lines. Cells were seeded onto either a PLLA SCAFFOLD (control) or PLLA SCAFFOLD coated with chamomile oil (experimental). A total of 24 SCAFFOLDs were inserted into the pancreatic area of each rabbit and placement was confirmed by ultrasound. After 21 days, immunohistochemistry analysis of INSULIN-producing like cells on protein levels confirmed INSULIN expression of INSULIN producing cells (IPSCs).Real-time polymerase chain reaction (PCR) determined the expressions of genes related to pancreatic endocrine development and function.Results: Fourier transform infrared spectroscopy (FTIR) results confirmed the existence of oil on the surface of the PLLA SCAFFOLD. The results showed a new peak at 2854 cm-1 for the aliphatic CH2 bond. Pdx1 expression was 0.051 ± 0.007 in the experimental group and 0.009 ± 0.002 in the control group. There was significantly increased INSULIN expression in the SCAFFOLD coated with chamomile oil (0.09 ± 0.001) compared to control group (0.063 ± 0.009, P≤.05). Both groups expressedNgn3 and Pdx1specific markers and pancreatic tissue was observed at 21 days post TRANSPLANTATION.Conclusion: The pancreatic region is an optimal site for DIFFERENTIATION of AMSCs to IPCs.Chamomile oil (as an antioxidant agent) can affect cell adhesion to the SCAFFOLD and increase cell DIFFERENTIATION. In addition, the oil may lead to increased blood glucose uptake in pathways in the muscles, liver and fatty tissue of a diabetic animal model by some probable molecular mechanisms.

Cites

The Protective Effects of Cultured Mesenchymal Stem Cells onto the Surface of Electrospun Poly-L-Lactide Acid Scaffolds Coated with Matricaria Chamomilla L. Oil in Streptozotocin-Induced Diabetic Rabbits

References

ADSCS ON PLLA/PCL HYBRID NANOSCAFFOLD AND GELATIN MODIFICATION: CYTOCOMPATIBILITY AND MECHANICAL PROPERTIES

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APA: Copy

FAZILI, AFSANEH, GHOLAMI, SOGHRA, MINAIE ZANGI, BAGHER, SEYEDJAFARI, EHSAN, & GHOLAMI, MAHDI. (2016). IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL. CELL JOURNAL (YAKHTEH), 18(3 (71)), 310-321. SID. https://sid.ir/paper/681576/en

Vancouver: Copy

FAZILI AFSANEH, GHOLAMI SOGHRA, MINAIE ZANGI BAGHER, SEYEDJAFARI EHSAN, GHOLAMI MAHDI. IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(3 (71)):310-321. Available from: https://sid.ir/paper/681576/en

IEEE: Copy

AFSANEH FAZILI, SOGHRA GHOLAMI, BAGHER MINAIE ZANGI, EHSAN SEYEDJAFARI, and MAHDI GHOLAMI, “IN VIVO DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS ON ELECTROSPUN POLY-L-LACTIDE ACID SCAFFOLDS COATED WITH MATRICARIA CHAMOMILLAL. OIL,” CELL JOURNAL (YAKHTEH), vol. 18, no. 3 (71), pp. 310–321, 2016, [Online]. Available: https://sid.ir/paper/681576/en

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