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Information Journal Paper

Title

CARBON NANOTUBE-ANANDAMIDE COMPLEX EXHIBITS SUSTAINED PROTECTIVE EFFECTS IN AN IN VITRO MODEL OF STROKE

Pages

  12-23

Abstract

 Introduction: The therapeutic potential of ANANDAMIDE (AEA) for the neurological disorders may be negatively affected by its short half-life or poor solubility. The superior properties of CARBON NANOTUBES (CNTs) for controlled drug delivery, prompted us to design AEA-CNTs complex and assess its effect in in vitro model of ISCHEMIC STROKE.Methods: In this experimental study, a multi-walled CNTs (MWCNTs) -AEA complex was prepared using amino-functionalized COOH-MWCNTs and characterized by Fourier transform infrared spectroscopy and transmission electron microscopy. PC12 cells in the presence of AEA (0.5, 1, 2 mg/ml), acid- or amine-modified MWCNTs, or MWCNTs-AEA complex (2, 5, 8 mg/ml) were exposed to 1 and 3 h oxygen-glucose deprivation (OGD) followed by 24 h re-oxygenation. In vitro cytotoxicity and OXIDATIVE STRESS were evaluated using three-way ANOVA.Results: AEA immobilization on the aminated MWCNTs was confirmed. OGD significantly reduced cell viability (P<0.001). After 3 h of OGD induction, COOHMWCNTs showed higher cytotoxicity than other MWCNTs (P<0.05, P<0.01, P<0.001) and MWCNTs-AEA was more protective than AEA alone (P<0.05, P<0.01). OGD increased malondialdehyde (MDA) and decreased glutathione (GSH) and superoxide dismutase (SOD) (P<0.001). Following 1-h OGD, AEA dose-dependently reduced MDA (P<0.001), and elevated GSH (P<0.05, P<0.01) and SOD (P<0.05, P<0.01), but AEA was ineffective following 3-h OGD (P>0.05). MWCNTs-AEA complex was effective at both time points (MDA and GSH: P<0.01, P<0.001, SOD: P<0.05, P<0.01, P<0.001). This nanostructure was more effective than AEA following longer exposure periods to OGD insult (P<0.05, P<0.01, P<0.001).Conclusion: Aminated MWCNTs are suitable carriers for AEA and provide longer -lasting effects against OGD insult.

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    Cite

    APA: Copy

    HASSANZADEH, PARICHEHR, ARBABI, ELHAM, ATYABI, FATEMEH, & DINARVAND, RASSOUL. (2016). CARBON NANOTUBE-ANANDAMIDE COMPLEX EXHIBITS SUSTAINED PROTECTIVE EFFECTS IN AN IN VITRO MODEL OF STROKE. PHYSIOLOGY AND PHARMACOLOGY, 20(1), 12-23. SID. https://sid.ir/paper/683570/en

    Vancouver: Copy

    HASSANZADEH PARICHEHR, ARBABI ELHAM, ATYABI FATEMEH, DINARVAND RASSOUL. CARBON NANOTUBE-ANANDAMIDE COMPLEX EXHIBITS SUSTAINED PROTECTIVE EFFECTS IN AN IN VITRO MODEL OF STROKE. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2016;20(1):12-23. Available from: https://sid.ir/paper/683570/en

    IEEE: Copy

    PARICHEHR HASSANZADEH, ELHAM ARBABI, FATEMEH ATYABI, and RASSOUL DINARVAND, “CARBON NANOTUBE-ANANDAMIDE COMPLEX EXHIBITS SUSTAINED PROTECTIVE EFFECTS IN AN IN VITRO MODEL OF STROKE,” PHYSIOLOGY AND PHARMACOLOGY, vol. 20, no. 1, pp. 12–23, 2016, [Online]. Available: https://sid.ir/paper/683570/en

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