THREE DIMENSIONAL CULTURING OF HUMAN JAW OSTEOBLASTS IN PLLA/HA SCAFFOLD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

FARYABI J.; NEMATOLLAHI S.N.; SALEHINIJAD P.; SHAHROKHI M.; KHORASANI M.T.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Kerman University of Medical Sciences Year:2013 | Volume:20 | Issue:5 Page(s): 435-449

Download Full-Text

Persian Verion

View:

600

Download:

Cites:

Information Journal Paper

Title

THREE DIMENSIONAL CULTURING OF HUMAN JAW OSTEOBLASTS IN PLLA/HA SCAFFOLD

Author(s)

Keywords

OSTEOBLASTSQ2

TISSUE ENGINEERINGQ2

PLLA POLYMERQ2

HYDROXYAPATITEQ2

TISSUE SCAFFOLDSQ2

Abstract

Background & Aims: TISSUE ENGINEERING using somatic cells and synthetic extracellular matrix (scaffold) represents a new approach for regeneration of mineralized tissue and bone. This study was carried out to investigate the ability of a PLLA/HA scaffold to culture osteoblast cells in a three dimensional milieu.Method: Three bony samples were taken from extraction sites during surgical extraction of wisdom teeth. OSTEOBLASTS were obtained from specimens by using trypsin and collagenase and were cultured in monolayer up to passage four. Cells were seeded on PLLA/HA scaffolds at density of 106 cells/ml and then incubated for 21 days. The seeded cells were evaluated by Hoechst, von kossa and H&E stainings and scanning electron microscopy.Results: Cellular growth was more pronounced when isolation was carried out by collagenase. According to scanning electron microscopy, osteoblast cells had been proliferated and attached to the scaffolds. H&E, Hoechst and von kossa stainings confirmed the presence of the harvested cells in the scaffold too.Conclusion: Our findings suggest that osteoblast cells can grow onto PLLA/HA scaffold in vitro.

Cites

No record.

References

No record.

Cite

APA: Copy

FARYABI, J., NEMATOLLAHI, S.N., SALEHINIJAD, P., SHAHROKHI, M., & KHORASANI, M.T.. (2013). THREE DIMENSIONAL CULTURING OF HUMAN JAW OSTEOBLASTS IN PLLA/HA SCAFFOLD. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, 20(5), 435-449. SID. https://sid.ir/paper/69/en

Vancouver: Copy

FARYABI J., NEMATOLLAHI S.N., SALEHINIJAD P., SHAHROKHI M., KHORASANI M.T.. THREE DIMENSIONAL CULTURING OF HUMAN JAW OSTEOBLASTS IN PLLA/HA SCAFFOLD. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;20(5):435-449. Available from: https://sid.ir/paper/69/en

IEEE: Copy

J. FARYABI, S.N. NEMATOLLAHI, P. SALEHINIJAD, M. SHAHROKHI, and M.T. KHORASANI, “THREE DIMENSIONAL CULTURING OF HUMAN JAW OSTEOBLASTS IN PLLA/HA SCAFFOLD,” JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, vol. 20, no. 5, pp. 435–449, 2013, [Online]. Available: https://sid.ir/paper/69/en

Related Journal Papers