COMPARISON OF BIOCOMPATIBILITY OF VARIOUS MEMBRANES WITH FIBROBLASTSY

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FOROUTAN TAHEREH; GHASEMI MAHMOUD; FARHANG SOLMAZ

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Journal Paper

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Journal: Journal of Dental School Year:2014 | Volume:32 | Issue:3 Page(s): 145-150

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Title

COMPARISON OF BIOCOMPATIBILITY OF VARIOUS MEMBRANES WITH FIBROBLASTSY

Author(s)

FOROUTAN TAHEREH | GHASEMI MAHMOUD | FARHANG SOLMAZ | Issue Writer Certificate

Keywords

ALKALINE PHOSPHATASE

FIBROBLAST

MTT

PRGF

Abstract

Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor (PRGF) at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament FIBROBLASTs (hPLF) cultured on Hypro-Sorb F, Pericardium and Tutodent resorbable membranes coated with PRGF.Methods: This experimental study was conducted on four resorbable membranes namely Hypro-Sorb F, Pericardium, Tutodent and Vicryl. FIBROBLAST cells isolated from the periodontal ligament of premolar tooth were passaged three times and 105 cells/ cm2 were cultured on membranes coated with PRGF. After 72 hours, the cells were evaluated in terms of biocompatibility and ALKALINE PHOSPHATASE (ALP) activity. Statistical analysis was carried out using one-way ANOVA.Results: PRGF increased cell adhesion and Tutodent membrane coated with PRGF showed the highest cell adhesion compared with Hypro-Sorb F and Pericardium membranes (p=0.005).Conclusion: PRGF increases cell viability and ALP activity of cells on biomembranes. PRGF treatment increases the adhesion of FIBROBLAST cells to these membranes.

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APA: Copy

FOROUTAN, TAHEREH, GHASEMI, MAHMOUD, & FARHANG, SOLMAZ. (2014). COMPARISON OF BIOCOMPATIBILITY OF VARIOUS MEMBRANES WITH FIBROBLASTSY. JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE, 32(3), 145-150. SID. https://sid.ir/paper/690825/en

Vancouver: Copy

FOROUTAN TAHEREH, GHASEMI MAHMOUD, FARHANG SOLMAZ. COMPARISON OF BIOCOMPATIBILITY OF VARIOUS MEMBRANES WITH FIBROBLASTSY. JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE[Internet]. 2014;32(3):145-150. Available from: https://sid.ir/paper/690825/en

IEEE: Copy

TAHEREH FOROUTAN, MAHMOUD GHASEMI, and SOLMAZ FARHANG, “COMPARISON OF BIOCOMPATIBILITY OF VARIOUS MEMBRANES WITH FIBROBLASTSY,” JOURNAL OF DENTAL SCHOOL SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCE, vol. 32, no. 3, pp. 145–150, 2014, [Online]. Available: https://sid.ir/paper/690825/en

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