RAPID DETECTION OF HIV-1 VIRAL RNA BY REAL-TIME TRANSCRIPTION MEDIATED AMPLIFICATION ASSAY

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PARYAN M.; MOHAMMADI YEGANEH S.; KHANSARINEJAD B.; MONDANIZADEH M.; PARYAN S.

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Journal: Journal of Arak University Medical Sciences Year:2012 | Volume:15 | Issue:4 (63) Page(s): 19-25

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Title

RAPID DETECTION OF HIV-1 VIRAL RNA BY REAL-TIME TRANSCRIPTION MEDIATED AMPLIFICATION ASSAY

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Keywords

HUMAN IMMUNODEFICIENCY VIRUS-1Q2

MOLECULAR BEACONQ2

PROBEQ2

TRANSCRIPTION MEDIATED AMPLIFICATIONQ2

Abstract

Background: Several different molecular methods have been developed that are capable of detecting HIV-1 in clinical specimens with different levels of sensitivity and specificity. This article describes the results of a reliability study on the development and application of a new real-time TMA method for isothermal detection of HIV-1.Materials and Methods: In this ex Primental study, the MOLECULAR BEACON primer and PROBE set were designed for a 176-base-pair region of HIV-1 pol gene using a specialized software.Logarithmic serial dilutions from 10-107 copies of an in-vitro transcribed RNA were used for determination of the analytical sensitivity of the assay. Clinical specimens that had previously been evaluated positive or negative by a valid commercial assay were used for assessing the clinical sensitivity and specificity of the assay.Results: The analytical and clinical sensitivities of the assay were determined 500 copies/ml and 93.3%, respectively. The primers and the PROBE were HIV-1 specific and no cross-reaction was observed with other blood-borne viruses and human genome bioinformatically. The clinical specificity of the developed real-time TMA assay was examined experimentally using 20 negative samples and determined to be 100%.Conclusion: The developed real-time TMA assay can be used as an appropriate tool for the rapid and isothermal detection of HIV-1 in patients' blood and plasma samples.

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APA: Copy

PARYAN, M., MOHAMMADI YEGANEH, S., KHANSARINEJAD, B., MONDANIZADEH, M., & PARYAN, S.. (2012). RAPID DETECTION OF HIV-1 VIRAL RNA BY REAL-TIME TRANSCRIPTION MEDIATED AMPLIFICATION ASSAY. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 15(4 (63)), 19-25. SID. https://sid.ir/paper/69683/en

Vancouver: Copy

PARYAN M., MOHAMMADI YEGANEH S., KHANSARINEJAD B., MONDANIZADEH M., PARYAN S.. RAPID DETECTION OF HIV-1 VIRAL RNA BY REAL-TIME TRANSCRIPTION MEDIATED AMPLIFICATION ASSAY. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2012;15(4 (63)):19-25. Available from: https://sid.ir/paper/69683/en

IEEE: Copy

M. PARYAN, S. MOHAMMADI YEGANEH, B. KHANSARINEJAD, M. MONDANIZADEH, and S. PARYAN, “RAPID DETECTION OF HIV-1 VIRAL RNA BY REAL-TIME TRANSCRIPTION MEDIATED AMPLIFICATION ASSAY,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 15, no. 4 (63), pp. 19–25, 2012, [Online]. Available: https://sid.ir/paper/69683/en

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