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Information Journal Paper

Title

Silibinin Inhibits TGF-β-induced MMP-2 and MMP-9 Through Smad Signaling Pathway in Colorectal Cancer HT-29 Cells

Pages

  81-90

Abstract

 Background: Metastasis of cancer cells is the primary responsible for death in patients with colorectal cancer (CRC). Transforming growth factor-β (TGF-β )-induced matrix metalloproteinases (MMPs) are essential for the metastasis process. Silibinin is a natural compound extracted from the Silybum marianum that exhibits anti-neoplastic activity in cancer cell lines. In this study, we evaluated the effects of Silibinin on MMP-2 and MMP-9 induced by TGF-β in human HT-29 CRC cell line and the potential mechanism underlying the effects. Methods: The present in vitro study was done on the HT-29 cell line. The HT-29 cell line was cultured in RPMI1640 and exposed to TGF-β (5 ng/ml) in the absence and presence of different concentrations of Silibinin (10, 25, 50, and 100 μ M). The effect of Silibinin on HT-29 cell viability was measured with the MTT assay. A real-time polymerase chain reaction (Real-Time PCR) determined the relative mRNA expression of MMP-2 and MMP-9. Western blotting was employed to examine MMP-2 and MMP 9 protein expression and Smad2 phosphorylation. Results: Silibinin inhibits cell viability of HT-29 cell line at 24 hours in a dose-dependent manner. TGF-β increased the mRNA and protein expression of MMP-2, MMP-9, and phosphorylated Smad2 compared to controls. Pharmacological inhibition with Silibinin markedly blocked TGF-β – induced MMP-2 and MMP-9 mRNA and protein expression and Smad2 phosphorylation. Conclusion: Silibinin decreased the cell viability of HT-29 cancer cells in a dose-dependent manner. Silibinin also inhibited TGF-β-stimulated MMP-2 and MMP-9 expression in HT-29 cells, possibly mediated with the Smad2 signaling pathway.

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References

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APA: Copy

ZARE, ZAHRA, Nayerpour dizaj, Tina, Lohrasbi, Armaghan, Sheikhalishahi, Zakieh Sadat, Asadi, Amirhooman, Zakeri, Mana, Hosseinabadi, Fahimeh, Abazari, Omid, ABBASI, MOJTABA, & Khanicheragh, Parisa. (2020). Silibinin Inhibits TGF-β-induced MMP-2 and MMP-9 Through Smad Signaling Pathway in Colorectal Cancer HT-29 Cells. BASIC & CLINICAL CANCER RESEARCH, 12(2), 81-90. SID. https://sid.ir/paper/697283/en

Vancouver: Copy

ZARE ZAHRA, Nayerpour dizaj Tina, Lohrasbi Armaghan, Sheikhalishahi Zakieh Sadat, Asadi Amirhooman, Zakeri Mana, Hosseinabadi Fahimeh, Abazari Omid, ABBASI MOJTABA, Khanicheragh Parisa. Silibinin Inhibits TGF-β-induced MMP-2 and MMP-9 Through Smad Signaling Pathway in Colorectal Cancer HT-29 Cells. BASIC & CLINICAL CANCER RESEARCH[Internet]. 2020;12(2):81-90. Available from: https://sid.ir/paper/697283/en

IEEE: Copy

ZAHRA ZARE, Tina Nayerpour dizaj, Armaghan Lohrasbi, Zakieh Sadat Sheikhalishahi, Amirhooman Asadi, Mana Zakeri, Fahimeh Hosseinabadi, Omid Abazari, MOJTABA ABBASI, and Parisa Khanicheragh, “Silibinin Inhibits TGF-β-induced MMP-2 and MMP-9 Through Smad Signaling Pathway in Colorectal Cancer HT-29 Cells,” BASIC & CLINICAL CANCER RESEARCH, vol. 12, no. 2, pp. 81–90, 2020, [Online]. Available: https://sid.ir/paper/697283/en

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