DUAL EFFECTS OF CELL FREE SUPERNATANTS FROM LACTOBACILLUS ACIDOPHILUS AND LACTOBACILLUS RHAMNOSUS GG IN REGULATION OF MMP-9 BY UP-REGULATING TIMP-1 AND DOWN-REGULATING CD147 IN PMADIFFERENTIATED THP-1 CELLS

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Maghsood Faezeh; MIRSHAFIEY ABBAS; M.Farahani Mohadese; MODARRESSI MOHAMMAD HOSSEIN; JAFARI PARVANEH; MOTEVASELI ELAHE

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Journal: Cell Journal (Yakhteh) Year:2018 | Volume:19 | Issue:4 Page(s): 559-568

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Title

DUAL EFFECTS OF CELL FREE SUPERNATANTS FROM LACTOBACILLUS ACIDOPHILUS AND LACTOBACILLUS RHAMNOSUS GG IN REGULATION OF MMP-9 BY UP-REGULATING TIMP-1 AND DOWN-REGULATING CD147 IN PMADIFFERENTIATED THP-1 CELLS

Author(s)

Keywords

CD147

INFLAMMATION

MMP

PROBIOTICS

TIMP

Abstract

Objective: Recent studies have reported dysregulated expression of matrix metalloproteinases (MMPs), especially MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1, -2(TIMP-1, TIMP-2), and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in activated macrophages of patients with inflammatory diseases. Therefore, MMP-2, MMP-9, and their regulators may represent a new target for treatment of inflammatory diseases. PROBIOTICS, which are comprised of lactic acid bacteria, have the potential to modulate inflammatory responses. In this experimental study, we investigated the anti-inflammatory effects of cell-free supernatants (CFS) fromLactobacillus acidophilus (L.acidophilus)and L. rhamnosus GG (LGG) in phorbol myristate acetate (PMA) -differentiated THP-1 cells.Materials and Methods: In this experimental study, PMA-differentiated THP-1 cells were treated with CFS from L.acidophilus, LGG and uninoculated bacterial growth media (as a control). The expression of MMP-2, MMP-9, TIMP-1, andTIMP-2 mRNAs were determined using real-time quantitative reverse transcription polymerase chain reaction (RTPCR).The levels of cellular surface expression of CD147 were assessed by flow cytometry, and the gelatinolytic activity of MMP-2 and MMP-9 were determined by zymography.Results: Our results showed that CFS from both L. acidophilus and LGG significantly inhibited the gene expression of MMP-9(P=0.0011 and P=0.0005, respectively), increased the expression of TIMP-1 (P<0.0001), decreased the cell surface expression of CD147 (P=0.0307 and P=0.0054, respectively), and inhibited the gelatinolytic activity of MMP-9 (P=0.0003 and P<0.0001, respectively) in PMA-differentiated THP-1 cells. Although, MMP-2 expression and activity and TIMP-2 expression remained unchanged.Conclusion: Our results indicate that CFS from L. acidophilus and LGG possess anti-inflammatory properties and can modulate the inflammatory response.

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APA: Copy

Maghsood, Faezeh, MIRSHAFIEY, ABBAS, M.Farahani, Mohadese, MODARRESSI, MOHAMMAD HOSSEIN, JAFARI, PARVANEH, & MOTEVASELI, ELAHE. (2018). DUAL EFFECTS OF CELL FREE SUPERNATANTS FROM LACTOBACILLUS ACIDOPHILUS AND LACTOBACILLUS RHAMNOSUS GG IN REGULATION OF MMP-9 BY UP-REGULATING TIMP-1 AND DOWN-REGULATING CD147 IN PMADIFFERENTIATED THP-1 CELLS. CELL JOURNAL (YAKHTEH), 19(4), 559-568. SID. https://sid.ir/paper/699064/en

Vancouver: Copy

Maghsood Faezeh, MIRSHAFIEY ABBAS, M.Farahani Mohadese, MODARRESSI MOHAMMAD HOSSEIN, JAFARI PARVANEH, MOTEVASELI ELAHE. DUAL EFFECTS OF CELL FREE SUPERNATANTS FROM LACTOBACILLUS ACIDOPHILUS AND LACTOBACILLUS RHAMNOSUS GG IN REGULATION OF MMP-9 BY UP-REGULATING TIMP-1 AND DOWN-REGULATING CD147 IN PMADIFFERENTIATED THP-1 CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2018;19(4):559-568. Available from: https://sid.ir/paper/699064/en

IEEE: Copy

Faezeh Maghsood, ABBAS MIRSHAFIEY, Mohadese M.Farahani, MOHAMMAD HOSSEIN MODARRESSI, PARVANEH JAFARI, and ELAHE MOTEVASELI, “DUAL EFFECTS OF CELL FREE SUPERNATANTS FROM LACTOBACILLUS ACIDOPHILUS AND LACTOBACILLUS RHAMNOSUS GG IN REGULATION OF MMP-9 BY UP-REGULATING TIMP-1 AND DOWN-REGULATING CD147 IN PMADIFFERENTIATED THP-1 CELLS,” CELL JOURNAL (YAKHTEH), vol. 19, no. 4, pp. 559–568, 2018, [Online]. Available: https://sid.ir/paper/699064/en

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