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Information Journal Paper

Title

EXPRESSION OF FOLLICULOGENESIS-RELATED GENES IN VITRIFIED HUMAN OVARIAN TISSUE AFTER TWO WEEKS IN VITRO CULTURE

Pages

  18-26

Abstract

 Objective: This study was designed to evaluate the effects of VITRIFICATION and in vitro culture of HUMAN ovarian tissue on the expression of oocytic and follicular cell-related genes.Materials and Methods: In this experimental study, ovarian tissue samples were obtained from eight transsexual women. Samples were cut into small fragments and were then assigned to vitrified and non-vitrified groups. In each group, some tissue fragments were divided into un-cultured and cultured (in α-MEM medium for 2 weeks) subgroups. The normality of follicles was assessed by morphological observation under a light microscope using hematoxylin and eosin (H& E) staining. Expression levels of factor in the germ line alpha (FIGLA), KIT ligand (KL), growth differentiation factor 9 (GDF-9) and follicle stimulating hormone receptor (FSHR) genes were quantified in both groups by real-time reverse transcriptase polymerase chain reaction (RT-PCR) at the beginning and the end of culture.Results: The percentage of normal follicles was similar between non-cultured vitrified and non-vitrified groups (P>0.05), however, cultured tissues had significantly fewer normal follicles than non-cultured tissues in both vitrified and non-vitrified groups (P<0.05). In both cultured groups the rate of primary and secondary follicles was significantly higher than non-cultured tissues (P<0.05). The expression of all examined genes was not significantly altered in both non-cultured groups. Whiles, in comparison with cultured tissues non-cultured tissues, the expression of FIGLA gene was significantly decreased, KL gene was not changed, GDF-9 and FSHR genes was significantly increased (P<0.05).Conclusion: HUMAN ovarian VITRIFICATION following in vitro culture has no impairing effects on follicle normality and development and expression of related-genes. However, in vitroculture condition has deleterious effects on normality of follicles.

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References

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APA: Copy

SHAMS MOFARAHE, ZAHRA, SALEHNIA, MOJDEH, GHAFFARI NOVIN, MAREFAT, GHORBANMEHR, NASSIM, & GHOLAMI FESHARAKI, MOHAMMAD. (2017). EXPRESSION OF FOLLICULOGENESIS-RELATED GENES IN VITRIFIED HUMAN OVARIAN TISSUE AFTER TWO WEEKS IN VITRO CULTURE. CELL JOURNAL (YAKHTEH), 19(1), 18-26. SID. https://sid.ir/paper/700132/en

Vancouver: Copy

SHAMS MOFARAHE ZAHRA, SALEHNIA MOJDEH, GHAFFARI NOVIN MAREFAT, GHORBANMEHR NASSIM, GHOLAMI FESHARAKI MOHAMMAD. EXPRESSION OF FOLLICULOGENESIS-RELATED GENES IN VITRIFIED HUMAN OVARIAN TISSUE AFTER TWO WEEKS IN VITRO CULTURE. CELL JOURNAL (YAKHTEH)[Internet]. 2017;19(1):18-26. Available from: https://sid.ir/paper/700132/en

IEEE: Copy

ZAHRA SHAMS MOFARAHE, MOJDEH SALEHNIA, MAREFAT GHAFFARI NOVIN, NASSIM GHORBANMEHR, and MOHAMMAD GHOLAMI FESHARAKI, “EXPRESSION OF FOLLICULOGENESIS-RELATED GENES IN VITRIFIED HUMAN OVARIAN TISSUE AFTER TWO WEEKS IN VITRO CULTURE,” CELL JOURNAL (YAKHTEH), vol. 19, no. 1, pp. 18–26, 2017, [Online]. Available: https://sid.ir/paper/700132/en

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