PROPAGATION OF CERTAIN ORNAMENTAL PLANTS BY TISSUE CULTURE

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MORTEZAEE NEIJAD F.; KHAVARINEZHAD RAMEZAN ALI

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Journal: JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY) Year:2004 | Volume:13 | Issue:50 Page(s): 4131-4140

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Information Journal Paper

Title

PROPAGATION OF CERTAIN ORNAMENTAL PLANTS BY TISSUE CULTURE

Author(s)

MORTEZAEE NEIJAD F. | KHAVARINEZHAD RAMEZAN ALI | Issue Writer Certificate

Keywords

BENZY/ADENINE BAQ4

DIMETHYL SULFOXIDE DMSOQ4

2Q4

4 DICHLOROPHENOXY ACETIC ACID 2-4D A NAPHTHALENE ACEFIC ACID NAAQ4

MURASHIGE AND SKOOG - MS MEDIUMQ4

Abstract

In this research the effects of plants growth regulator on the micropropagation of two ornamental flower Tulipa gesneriana and Lilium bulbiferum in a completely randomize block repeated three times. The common practice for wild and bread tulips is to plant the bulb and for Lilium to separate the green bulbs from the main. bulb. A variety of methods of micropropagation can be used to further reproduce these plants. In the first experiment conducted to plant the tulip seeds and to help callus formation, it was revealed that various 2,4-D concentrations significantly affect the rate of seed germination to the extent that a 10 milligram/Liter concentration of this hormone will produce maximal callus formation of germination in the dark, though it will not make a significant difference in the dark and in the light. To continue this experiment also conducted on tulip, to plant the pieces of the explanted tulip’s bulb scale using 10 mg/l 2,4-D hormone, it was revealed that the hormone signficantly affected the callus formation. Further, the effect of darkness and light was studies using this hormone, such that it was revealed that greatest callus formation occurs in the dark. In the second experiment where the pieces of the explanted Lilium bulb scale grew in the base culture medium with 5 mg/l benzyl-adenine BA, the surface of the planted pieces revealed shoot germination which on cold treatment and recuiture in a medium containing dimethyl sulphoxide DMSO, produced stems. To the continue research on the Lilium, the combined effect of Naphthalene acetic acid NAA and of benzyl adenine BA on the pieces of explanted bulb scale, revealed that a great quantity of callus was formed in the base medium containing 5 mg/lit of this hormone significantly affected the callus formation, where the individual effect of NAA and 2,4-D hormones studies, revealed that the varied concentrations of NAA in the culture medium, produced more callusd than for the control, such that the greatest callus formation occurred in the 2.5 milligram/Liter concentrations. On Further experimentation, the effects of 2, 4-D alone on the pieces of the Lilium explanted bulb scale revealed the greatest quantity of callus formation in a base medium containing 2.5 milligram/liter of 2.4-D. Further, on comparing 2, 4-D and NAA, it was revealed that NAA has a greater effects on callus formation.

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APA: Copy

MORTEZAEE NEIJAD, F., & KHAVARINEZHAD, RAMEZAN ALI. (2004). PROPAGATION OF CERTAIN ORNAMENTAL PLANTS BY TISSUE CULTURE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), 13(50), 4131-4140. SID. https://sid.ir/paper/70448/en

Vancouver: Copy

MORTEZAEE NEIJAD F., KHAVARINEZHAD RAMEZAN ALI. PROPAGATION OF CERTAIN ORNAMENTAL PLANTS BY TISSUE CULTURE. JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY)[Internet]. 2004;13(50):4131-4140. Available from: https://sid.ir/paper/70448/en

IEEE: Copy

F. MORTEZAEE NEIJAD, and RAMEZAN ALI KHAVARINEZHAD, “PROPAGATION OF CERTAIN ORNAMENTAL PLANTS BY TISSUE CULTURE,” JOURNAL OF SCIENCES (ISLAMIC AZAD UNIVERSITY), vol. 13, no. 50, pp. 4131–4140, 2004, [Online]. Available: https://sid.ir/paper/70448/en

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