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Information Journal Paper

Title

Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR

Pages

  98-103

Abstract

 Introduction: Leptospirosis is a significant public health problem in the Caspian littoral of Iran comprising Gilan, Mazandaran, and Golestan provinces. In Golestan province, serology assays indicated anti-Leptospira antibodies in animals and humans; however, no reliable record of infections in patients with signs and symptoms of the disease is available. Methods: We employed the indirect immunofluorescent antibody assay (IFA), and two PCR assays, a realtime PCR (qPCR) and a nested-PCR targeting 16S rDNA (rrs) sequence for Diagnosis of Leptospirosis in febrile patients in Golestan province. Results: Out of 52 febrile patients, 25 (48. 07%) had antibody titers ≥ 1/80 by IFA, and were defined as positive. In 7, 9, and 7 individuals, the antibody titers were 1/40, 1/20, and 1/10, respectively, and 4 had no antibodies. The qPCR and nested PCR detected leptospiral DNA in 55. 75% and 67. 3% of the patients' sera, respectively. The two PCR assays had a Kappa agreement of 0. 53 (P< 0. 0001), suggesting a moderate agreement, and showed a significant reverse association with the IFA titers (P<0. 05). Conclusion: Our results suggest that higher antibody titers accompanied the spirochete's removal from blood by the patient's immune response. Hence, a reliable Diagnosis of Leptospira infection necessitates deploying a DNA-based method alongside serology.

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    Cite

    APA: Copy

    NADDAF, SAIED REZA, MOHABATI, REYHANEH, Vahabpor, Rouhollah, Naeimi, Sabah, & EYBPOOSH, SANA. (2020). Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, 8(3), 98-103. SID. https://sid.ir/paper/705620/en

    Vancouver: Copy

    NADDAF SAIED REZA, MOHABATI REYHANEH, Vahabpor Rouhollah, Naeimi Sabah, EYBPOOSH SANA. Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR. JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES[Internet]. 2020;8(3):98-103. Available from: https://sid.ir/paper/705620/en

    IEEE: Copy

    SAIED REZA NADDAF, REYHANEH MOHABATI, Rouhollah Vahabpor, Sabah Naeimi, and SANA EYBPOOSH, “Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR,” JOURNAL OF MEDICAL MICROBIOLOGY AND INFECTIOUS DISEASES, vol. 8, no. 3, pp. 98–103, 2020, [Online]. Available: https://sid.ir/paper/705620/en

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