UPREGULATION OF INTEGRIN-α6 AND INTEGRIN-β1 GENE EXPRESSIONS IN MOUSE SPERMATOGONIAL STEM CELLS AFTER CONTINUES AND PULSED LOW INTENSITY ULTRASOUND STIMULATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAQIQ MAHDI; MOVAHEDIN MANSOUREH; MOKHTARI DIZAJI MANIJHE; MAZAHERI ZOHREH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2018 | Volume:19 | Issue:4 Page(s): 634-639

Download Full-Text

View:

264

Download:

197

Cites:

Information Journal Paper

Title

UPREGULATION OF INTEGRIN-α6 AND INTEGRIN-β1 GENE EXPRESSIONS IN MOUSE SPERMATOGONIAL STEM CELLS AFTER CONTINUES AND PULSED LOW INTENSITY ULTRASOUND STIMULATION

Author(s)

MOHAQIQ MAHDI | MOVAHEDIN MANSOUREH | MOKHTARI DIZAJI MANIJHE | MAZAHERI ZOHREH | Issue Writer Certificate

Keywords

COLONIZATION

PROLIFERATION

STEM CELL

ULTRASOUND

Abstract

Objective: low intensity ULTRASOUND (continues and pulsed) is a form of energy. Spermatogonial STEM CELLs (SSCs) are at the base of male fertility. This study investigated the effects of low intensity ULTRASOUND stimulation (LIUS) and low intensity pulsed ULTRASOUND stimulation (LIUPS) on the expression of germ cell-specific and pluripotency genes in SSCs in vitro.Materials and Methods: In this experimental study, isolated SSCs from neonatal male mice were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). In addition, to confirm identification of SSCs, PLZF protein was detected positively in SSCs derived colonies. SSCs were stimulated by LIUS and LIUPS for 5 days, followed by assessment of expression ofintegrin-α6 (Itga6) and β1 (Itgβ1), as two germ cell-specific genes, and Oct-4, as a pluripotency gene, on day 21st by quantitive reverse transcriptase-polymerase chain reaction (qRT-PCR). To investigate the PROLIFERATION rate and COLONIZATION of SSCs in different groups, counting whole number of the cells and colonies as well as analysis of the respective diameters were performed on days 7th, 14th and 21st. Data was analyzed by ANOVA test.Results: LIUS and LIUPS treatment of mouse SSCs increased expression of Itga6 and Itgβ1 genes in the experimental groups, compared to the control group (P<0.05), whereas there was no significant difference between the groups, regarding the expression ofOct-4 gene. These treatments maintained survival rate, while they increased PROLIFERATION rate and COLONIZATION of SSCs during the first week of culture. However, within the second week, PROLIFERATION rate and COLONIZATION were decreased in the experimental groups.Conclusion: These results suggested that LIUS and LIUPS treatment had good effect on SSCs PROLIFERATION and COLONIZATION, based on the gene-specific marker expression during 21 days culturein vitro.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAQIQ, MAHDI, MOVAHEDIN, MANSOUREH, MOKHTARI DIZAJI, MANIJHE, & MAZAHERI, ZOHREH. (2018). UPREGULATION OF INTEGRIN-α6 AND INTEGRIN-β1 GENE EXPRESSIONS IN MOUSE SPERMATOGONIAL STEM CELLS AFTER CONTINUES AND PULSED LOW INTENSITY ULTRASOUND STIMULATION. CELL JOURNAL (YAKHTEH), 19(4), 634-639. SID. https://sid.ir/paper/706149/en

Vancouver: Copy

MOHAQIQ MAHDI, MOVAHEDIN MANSOUREH, MOKHTARI DIZAJI MANIJHE, MAZAHERI ZOHREH. UPREGULATION OF INTEGRIN-α6 AND INTEGRIN-β1 GENE EXPRESSIONS IN MOUSE SPERMATOGONIAL STEM CELLS AFTER CONTINUES AND PULSED LOW INTENSITY ULTRASOUND STIMULATION. CELL JOURNAL (YAKHTEH)[Internet]. 2018;19(4):634-639. Available from: https://sid.ir/paper/706149/en

IEEE: Copy

MAHDI MOHAQIQ, MANSOUREH MOVAHEDIN, MANIJHE MOKHTARI DIZAJI, and ZOHREH MAZAHERI, “UPREGULATION OF INTEGRIN-α6 AND INTEGRIN-β1 GENE EXPRESSIONS IN MOUSE SPERMATOGONIAL STEM CELLS AFTER CONTINUES AND PULSED LOW INTENSITY ULTRASOUND STIMULATION,” CELL JOURNAL (YAKHTEH), vol. 19, no. 4, pp. 634–639, 2018, [Online]. Available: https://sid.ir/paper/706149/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops