HAIRY ROOT CULTURES OF HYPERICUM PERFORATUM L., A PROMISING METHOD FOR THE HIGH SCALE PRODUCTION OF HYPERICIN

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MONTAZERI M.; AZADBAKHT M.; NAJAFI ZARRINI H.; NEMATZADEH GH.A.; PAKDIN PARIZI A.; DAVOODI A.

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Journal Paper

Paper Information

Journal: Journal of Medicinal Plants Year:2018 | Volume:17 | Issue:67 Page(s): 55-67

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Title

HAIRY ROOT CULTURES OF HYPERICUM PERFORATUM L., A PROMISING METHOD FOR THE HIGH SCALE PRODUCTION OF HYPERICIN

Author(s)

Keywords

HYPERICUM PERFORATUM

RHIZOBIUM RHIZOGENES

HAIRY ROOT

HPLC

HYPERICIN

Abstract

Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. HAIRY ROOT cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites.Objective: In this study, HAIRY ROOTs of HYPERICUM PERFORATUM have been prepared using the seeds and bio transformed by bacteria. Finally, the HYPERICIN have been producted by the HAIRY ROOTs.Methods: First, the seeds have been incubated in the plant media to HAIRY ROOTs produced. Then, the HAIRY ROOTs have been dipped in Rhizobium rizogenes suspension for biotransformation of bacterial genes. Morphological and phytochemical features of HAIRY ROOTs have been determined in order to select the H. perforatum genotypes with higher HYPERICIN contents. The fresh and dry weight of ten lines clones were measured after 30 days. In addition to, methanolic extracts of final HAIRY ROOTs have been prepared and HYPERICIN has been isolated and assayed by High Performance Liquid Chromatography.Results: According to the observations, one of ten HAIRY ROOT lines shows 75-fold higher HYPERICIN content (339.27 ppm) compared to non-transformedH. perforatum (4.58 ppm) in the same of body weight of roots. The clone with the most content of HYPERICIN had significant development of biomass of HAIRY ROOTs and increase the HYPERICIN production. Transformed clones were varied in morphology, growth, and metabolite productivity.Conclusion: The mentioned methods induce the production of HAIRY ROOT secondary metabolites in high scale to improve the quality and the quantity of pharmaceutical compounds.

Cites

Plant cells technology as an effective biotechnological approach for high scale production of pharmaceutical natural compounds: A meta-analysis study

References

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APA: Copy

MONTAZERI, M., AZADBAKHT, M., NAJAFI ZARRINI, H., NEMATZADEH, GH.A., PAKDIN PARIZI, A., & DAVOODI, A.. (2018). HAIRY ROOT CULTURES OF HYPERICUM PERFORATUM L., A PROMISING METHOD FOR THE HIGH SCALE PRODUCTION OF HYPERICIN. JOURNAL OF MEDICINAL PLANTS, 17(67), 55-67. SID. https://sid.ir/paper/709044/en

Vancouver: Copy

MONTAZERI M., AZADBAKHT M., NAJAFI ZARRINI H., NEMATZADEH GH.A., PAKDIN PARIZI A., DAVOODI A.. HAIRY ROOT CULTURES OF HYPERICUM PERFORATUM L., A PROMISING METHOD FOR THE HIGH SCALE PRODUCTION OF HYPERICIN. JOURNAL OF MEDICINAL PLANTS[Internet]. 2018;17(67):55-67. Available from: https://sid.ir/paper/709044/en

IEEE: Copy

M. MONTAZERI, M. AZADBAKHT, H. NAJAFI ZARRINI, GH.A. NEMATZADEH, A. PAKDIN PARIZI, and A. DAVOODI, “HAIRY ROOT CULTURES OF HYPERICUM PERFORATUM L., A PROMISING METHOD FOR THE HIGH SCALE PRODUCTION OF HYPERICIN,” JOURNAL OF MEDICINAL PLANTS, vol. 17, no. 67, pp. 55–67, 2018, [Online]. Available: https://sid.ir/paper/709044/en

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