DETECTION OF NEISSERIA MENINGITIDIS IN PEDIATRICS MENINGITIS BY PCR, IN COMPARISON TO CULTURE AND AGGLUTINATION TESTS (SHORT REPORT)

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GHOTASLOU R.; FARAJNIA S.

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Paper Information

Journal: Journal of Rafsanjan University of Medical Sciences Year:2013 | Volume:11 | Issue:6 (47) Page(s): 605-610

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Title

DETECTION OF NEISSERIA MENINGITIDIS IN PEDIATRICS MENINGITIS BY PCR, IN COMPARISON TO CULTURE AND AGGLUTINATION TESTS (SHORT REPORT)

Author(s)

GHOTASLOU R. | FARAJNIA S. | Issue Writer Certificate

Keywords

MENINGITISQ2

NEISSERIA MENINGITIDISQ3

PCRQ2

Abstract

Background and Objectives: Bacterial MENINGITIS especially meningococcal MENINGITIS is a serious and sometimes fatal infection. The aim of this study was the determination of MENINGITIS disease by routine and molecular assays.Materials and Methods: CSF samples were collected from 275 children and agglutination, bacteriological tests were achieved. DNA extraction was done according to phenol-chlorophorm method and PCR was carried out by the specific primers.Results: The mean age of the patients was 42±2 month. Younges was 11 days and the oldest child was 11 years old. Meningococcal MENINGITIS was more common in the male gender. Totally, NEISSERIA MENINGITIDIS was found in 3 (1.1%), 5 (1.8%) and 10 (3.6%) by culture, agglutination and PCR assays, respectively.Conclusion: One of the most common etiological agents of MENINGITIS is NEISSERIA MENINGITIDIS in the Azerbaijan state. Molecular diagnosis has higher sensitivity and specify in comparison to culture and agglutination tests.

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APA: Copy

GHOTASLOU, R., & FARAJNIA, S.. (2013). DETECTION OF NEISSERIA MENINGITIDIS IN PEDIATRICS MENINGITIS BY PCR, IN COMPARISON TO CULTURE AND AGGLUTINATION TESTS (SHORT REPORT). JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 11(6 (47)), 605-610. SID. https://sid.ir/paper/71143/en

Vancouver: Copy

GHOTASLOU R., FARAJNIA S.. DETECTION OF NEISSERIA MENINGITIDIS IN PEDIATRICS MENINGITIS BY PCR, IN COMPARISON TO CULTURE AND AGGLUTINATION TESTS (SHORT REPORT). JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2013;11(6 (47)):605-610. Available from: https://sid.ir/paper/71143/en

IEEE: Copy

R. GHOTASLOU, and S. FARAJNIA, “DETECTION OF NEISSERIA MENINGITIDIS IN PEDIATRICS MENINGITIS BY PCR, IN COMPARISON TO CULTURE AND AGGLUTINATION TESTS (SHORT REPORT),” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 11, no. 6 (47), pp. 605–610, 2013, [Online]. Available: https://sid.ir/paper/71143/en

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