THE EFFECT OF GARLIC EXTRACT ON EXPRESSION OF IFN-γ AND INOS GENES IN MACROPHAGES INFECTED WITH LEISHMANIA MAJOR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHARAVI M.J.; NOBAKHT M.; KHADEMVATAN SH.; BANDANI E.; ROUZBAHANI M.; BAKHSHAYESH M.; SAMADI KOUCHAKSARAEI A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES Year:2012 | Volume:11 | Issue:4 (45) Page(s): 313-322

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,021

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF GARLIC EXTRACT ON EXPRESSION OF IFN-γ AND INOS GENES IN MACROPHAGES INFECTED WITH LEISHMANIA MAJOR

Author(s)

Keywords

AQUEOUS GARLIC EXTRACT (AGE)Q2

IFNγ AND INOS GENESQ2

LEISHMANIA MAJORQ2

J774 CELL LINEQ2

Abstract

Background and Objectives: The protozoa of leishmania lead to cutaneus and visceral leishmaniasis.Activation macrophages phagocyte parasites and this mechanism can be done with production of active radicals oxygen and nitric oxide, That is done through immunomodulator, as a basic process for production of the new drug. In this study, the effect of garlic extract as an immunomodulator on J774 cells infected with LEISHMANIA MAJOR and iNOS gene expression and IFNg has been investigated.Materials and Methods: LEISHMANIA MAJOR promastigotes (MRHO/IR/75/ER) were added to the in-vitro cultured macrophages (J774 cells) and these cells were incubated for 72 hours. Various concentrations of garlic extract (9.25-18.5-37-74-148 mg/ml) were added to the infected cells. MTT assay was applied for cellular prolifration. After 72 hours of incubation, supernatants were collected and total RNA was extracted from the infected cells. The expression of IFNg and INOS genes were studied by RT-PCR method. Untreated sample with garlic was used as control. All the experiments were repeated at least three times, and representative results were analyzed. Statistical significance (P<0.05) was analyzed by Student’s t -test using SPSS version16.Results: The result of this study, using MTT method showed that, the IC50 concentration of garlic extract was 37 mg/ml. Also the expression of IFNg and iNOS genes by RT-PCR indicated that garlic extract lead to rise of expression of these genes in the J774 cells infected with L.major.Conclusion: Based on the findings and importance of the cellular immunity cytokines in developing and importance of the responses, the hypothesis that the effect of AGE in cellular immunity is due to rising the IFNγ cytokines and expression of iNOS genes is confirmed.

Cites

No record.

References

No record.

Cite

APA: Copy

GHARAVI, M.J., NOBAKHT, M., KHADEMVATAN, SH., BANDANI, E., ROUZBAHANI, M., BAKHSHAYESH, M., & SAMADI KOUCHAKSARAEI, A.. (2012). THE EFFECT OF GARLIC EXTRACT ON EXPRESSION OF IFN-γ AND INOS GENES IN MACROPHAGES INFECTED WITH LEISHMANIA MAJOR. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 11(4 (45)), 313-322. SID. https://sid.ir/paper/71172/en

Vancouver: Copy

GHARAVI M.J., NOBAKHT M., KHADEMVATAN SH., BANDANI E., ROUZBAHANI M., BAKHSHAYESH M., SAMADI KOUCHAKSARAEI A.. THE EFFECT OF GARLIC EXTRACT ON EXPRESSION OF IFN-γ AND INOS GENES IN MACROPHAGES INFECTED WITH LEISHMANIA MAJOR. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2012;11(4 (45)):313-322. Available from: https://sid.ir/paper/71172/en

IEEE: Copy

M.J. GHARAVI, M. NOBAKHT, SH. KHADEMVATAN, E. BANDANI, M. ROUZBAHANI, M. BAKHSHAYESH, and A. SAMADI KOUCHAKSARAEI, “THE EFFECT OF GARLIC EXTRACT ON EXPRESSION OF IFN-γ AND INOS GENES IN MACROPHAGES INFECTED WITH LEISHMANIA MAJOR,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 11, no. 4 (45), pp. 313–322, 2012, [Online]. Available: https://sid.ir/paper/71172/en

Related Journal Papers