COMPARISON OF IN VITRO CYTOTOXIC EFFECT OF TWO MTAS (IRAN MADE AND IMPORTED) ON PERIPHERAL BLOOD MONONUCLEAR CELLS

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NAZARI MOGHADAM K.; GHAZANFARI T.; MOHAMMADI BASIR M.; EMADI M.

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Paper Information

Journal: Journal of Rafsanjan University of Medical Sciences Year:2006 | Volume:4 | Issue:4-B (17) Page(s): 320-325

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Information Journal Paper

Title

COMPARISON OF IN VITRO CYTOTOXIC EFFECT OF TWO MTAS (IRAN MADE AND IMPORTED) ON PERIPHERAL BLOOD MONONUCLEAR CELLS

Author(s)

NAZARI MOGHADAM K. | GHAZANFARI T. | MOHAMMADI BASIR M. | EMADI M. | Issue Writer Certificate

Keywords

ROOT MTAQ4

MTAQ3

CELL CYTOTOXICITYQ2

MTT ASSAYQ2

Abstract

Background and Objective: Imported mineral Trioxide Aggregate (MTA) as a root –end filling material has many applications in root repairing and bone healing including direct pulp capping, repair of root and furcation perforation, and apexification. By introducing a similar material, made in Iran, namely MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA, primary tests such as cytotoxicity test should be performed. The purpose of this study was to compare the 24h and 48h cytotoxicity effect of MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA on peripheral blood mononuclear cells using MTT ASSAY. Materials and Methods: In this study, after blood sampling, peripheral blood mononuclear cells were isolated on a gradient of ficole hypac. After several washing phases, cells were cultured in RPMI with 10% FCS in 96 well U shape microtiterplates in a 5% Co2 incubator. Serial dilution of MTA and MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA were added to the wells. CELL CYTOTOXICITY was determined, 24 and 48 hours after treatment by MTT ASSAY. Results: Cytotoxicity of MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA was less than that of MTA (ProRoot ). At 24 hours after culture there was significant difference between cell viability affected by MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA and MTA in the three different dilutions 20 λ, 50 λ, and 100 λ (p<0.05). At 48 hours, there was also a significant difference between cell viability after using MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA and MTA in two dilutions; 50 λ, 100 λ (p<0.05). Conclusion: MTA/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">ROOT MTA could be considered as a proper alternative for MTA. For ther studies such as clinical trials should also be performed.

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References

TISSUE REACTION TO MTA AND PORTLAND CEMENT IN RAT

Cite

APA: Copy

NAZARI MOGHADAM, K., GHAZANFARI, T., MOHAMMADI BASIR, M., & EMADI, M.. (2006). COMPARISON OF IN VITRO CYTOTOXIC EFFECT OF TWO MTAS (IRAN MADE AND IMPORTED) ON PERIPHERAL BLOOD MONONUCLEAR CELLS. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 4(4-B (17)), 320-325. SID. https://sid.ir/paper/71397/en

Vancouver: Copy

NAZARI MOGHADAM K., GHAZANFARI T., MOHAMMADI BASIR M., EMADI M.. COMPARISON OF IN VITRO CYTOTOXIC EFFECT OF TWO MTAS (IRAN MADE AND IMPORTED) ON PERIPHERAL BLOOD MONONUCLEAR CELLS. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2006;4(4-B (17)):320-325. Available from: https://sid.ir/paper/71397/en

IEEE: Copy

K. NAZARI MOGHADAM, T. GHAZANFARI, M. MOHAMMADI BASIR, and M. EMADI, “COMPARISON OF IN VITRO CYTOTOXIC EFFECT OF TWO MTAS (IRAN MADE AND IMPORTED) ON PERIPHERAL BLOOD MONONUCLEAR CELLS,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 4, no. 4-B (17), pp. 320–325, 2006, [Online]. Available: https://sid.ir/paper/71397/en

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