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Information Journal Paper

Title

EFFECTS OF RE-VITRIFICATION OF MOUSE MORULA AND EARLY BLASTOCYST STAGES ON APOPTOTIC GENE EXPRESSION AND DEVELOPMENTAL POTENTIAL

Pages

  614-619

Keywords

Abstract

 Objective: Re-vitrification of EMBRYOs immediately after thawing or after a culture period could be used to preserve the extra EMBRYOs after EMBRYO transfer. This study aims to clarify the effect of re-vitrification onBax and Bcl-2 gene expressions of compact and early blastocyst stage EMBRYOs.Materials and Methods: This experimental study was performed on MOUSE EMBRYOs. We collected 8-cell stage EMBRYOs (n=400) from female mature mice, 60-62 hoursafter injection of human chorionic gonadotropin (hCG). The EMBRYOs were divided into 5 groups: fresh (n=80), vitrified at the 8-cell stage (n=80), vitrified at the blastocyst stage (n=80), vitrified at the 8-cell stage, and re-vitrified at the compact (n=80) and early blastocyst stages (n=80). EMBRYOs were vitrified by cryolock. We analyzed the developmental rates of the vitrified-warmed EMBRYOs with the chi-square test. Quantitative polymerase chain reaction (qPCR) data were analyzed with SPSS version 16 using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. P<0.05 were considered statistically significant.Results: The expanded blastocyst formation rate showed a significant difference in re-vitrified EMBRYOs compared with fresh EMBRYOs (P<0.05). However, this result was similar between the two re-vitrified groups. Our data showed a significant difference in expression of theBax and Bcl-2 GENES between re-vitrified and fresh EMBRYOs (P<0.05).Expressions of theBax and Bcl-2 GENES showed no significant difference between the two re-vitrified groups.Conclusion: Based on our study, re-vitrification could affect developmental rate and expressions of the Bax and Bcl2 GENES.

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    APA: Copy

    MAJIDI GHARENAZ, NASRIN, MOVAHEDIN, MANSOUREH, & MAZAHERI, ZOHREH. (2018). EFFECTS OF RE-VITRIFICATION OF MOUSE MORULA AND EARLY BLASTOCYST STAGES ON APOPTOTIC GENE EXPRESSION AND DEVELOPMENTAL POTENTIAL. CELL JOURNAL (YAKHTEH), 19(4), 614-619. SID. https://sid.ir/paper/720449/en

    Vancouver: Copy

    MAJIDI GHARENAZ NASRIN, MOVAHEDIN MANSOUREH, MAZAHERI ZOHREH. EFFECTS OF RE-VITRIFICATION OF MOUSE MORULA AND EARLY BLASTOCYST STAGES ON APOPTOTIC GENE EXPRESSION AND DEVELOPMENTAL POTENTIAL. CELL JOURNAL (YAKHTEH)[Internet]. 2018;19(4):614-619. Available from: https://sid.ir/paper/720449/en

    IEEE: Copy

    NASRIN MAJIDI GHARENAZ, MANSOUREH MOVAHEDIN, and ZOHREH MAZAHERI, “EFFECTS OF RE-VITRIFICATION OF MOUSE MORULA AND EARLY BLASTOCYST STAGES ON APOPTOTIC GENE EXPRESSION AND DEVELOPMENTAL POTENTIAL,” CELL JOURNAL (YAKHTEH), vol. 19, no. 4, pp. 614–619, 2018, [Online]. Available: https://sid.ir/paper/720449/en

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