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Information Journal Paper

Title

Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China

Pages

  106-111

Abstract

 Objective(s): Acinetobacter pittii has become an emerging opportunistic noscomial pathogen worldwide with multi-drug resistance. In the present study, an A. pittii strain was isolated from bronchoalveolar lavage fluid sample harboring both OXA-58 and NDM-1Carbapenemase producing genes. The mechanisms of carbapenem resistance of the A. pittii strain was investigated. Materials and Methods: Carbapenemase producing genes were examined by PCR and DNA sequencing. S1-PFGE was used to localize Carbapenemase encoding genes. Filter mating and electrotransformation were used to investigate the transferability of such Carbapenemase encoding genes between different strains. Genetic surroundings of blaOXA-58 and blaNDM-1 genes were detected as well. Results: The A. pittii strain, carrying both OXA-58 and NDM-1 Carbapenemase encoding genes, was resistant to all β-lactam antibiotics, while suscepitible to ciprofloxacin, levofloxacin, tobramycin, cotrimoxazole and tigecycline. Southern blot hybridization for the blaOXA-58 and blaNDM-1 gene indicated that the two genes locate in the same plasmid with molecular weight of 310. 1-336. 5kb. BlaOXA-58 was located in an ISAba3-blaOXA-58-ISAba3-like structure, and the blaNDM-1 gene cluster was embedded in an ISAba125-aphA6-blaNDM-1-bleMBL-Δ trpF-dsbC-cutA structure sequentially. Conclusion: In the present study, it is first reported an A. pittii clincal strain in China, Co-harboring OXA-58 and NDM-1 Carbapenemase producing genes residing on a same plasmid. In hospital and community settings, it is of great significance and urgence to increase the surveillance of these kinds of organisms.

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    • Cite

    APA: Copy

    Chen, Yili, Guo, Penghao, Huang, Han, Huang, Yongxin, Wu, Zhongwen, & Liao, Kang. (2019). Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(1), 106-111. SID. https://sid.ir/paper/721619/en

    Vancouver: Copy

    Chen Yili, Guo Penghao, Huang Han, Huang Yongxin, Wu Zhongwen, Liao Kang. Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(1):106-111. Available from: https://sid.ir/paper/721619/en

    IEEE: Copy

    Yili Chen, Penghao Guo, Han Huang, Yongxin Huang, Zhongwen Wu, and Kang Liao, “Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 1, pp. 106–111, 2019, [Online]. Available: https://sid.ir/paper/721619/en

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