OPTIMIZATION OF CATALYTIC ACTIVITY OF PHYTASE FROM ISOLATE K46B USING RESPONSE SURFACE METHODOLOGY

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ROCKY SALIMI K.; HASHEMI M.; SAFARI M.

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Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2015 | Volume:13 | Issue:1 (SPECIAL ISSUE) Page(s): 203-215

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Title

OPTIMIZATION OF CATALYTIC ACTIVITY OF PHYTASE FROM ISOLATE K46B USING RESPONSE SURFACE METHODOLOGY

Author(s)

ROCKY SALIMI K. | HASHEMI M. | SAFARI M. | Issue Writer Certificate

Keywords

PHYTASE ENZYMEQ2

SUBMERGED FERMENTATIONQ2

CATALYTIC ACTIVITYQ2

OPTIMIZATIONQ2

RESPONSE SURFACE METHODOLOGYQ1

Abstract

Phytases (myo-inositol 1, 2, 3, 4, 5, 6 hexakis phospho-hydrolase), found in plants, animal tissues and microorganisms, are a group of phosphatases capable of hydrolyzing phytic acid, the most abundant inositiol phosphate in nature, to myo-inositol and inorganic phosphates. In this study, 68 microbial isolates from different sources were screened using SUBMERGED FERMENTATION for the best phytase production. The results showed that isolate K46b had the highest phytase production (1.952 U/mL) among other isolates. Subsequently, the CATALYTIC ACTIVITY of PHYTASE ENZYME of isolate K46b at different conditions of temperature, pH and sodium phytate concentration was optimized using RESPONSE SURFACE METHODOLOGY (RSM). Under optimum conditions i.e.56.5 ºC, pH 7.30 and 2.05 mM sodium phytate, the phytase activity increased to 4.627 U/mL which compared to the screening step, it showed a 137% increase. Moreover, the phytase showed 60-73% of its optimum activity in wide ranges of temperature (47-68 ºC), pH (6.3-8.0) and sodium phytate (1.04-2.50 mM). It can be concluded that isolate K46b phytase has potential applications in dephytinization of food ingredients such as cereals and meals in in food and animal feed industries, aquaculture and combating phosphorous pollution in the environment.

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APA: Copy

ROCKY SALIMI, K., HASHEMI, M., & SAFARI, M.. (2015). OPTIMIZATION OF CATALYTIC ACTIVITY OF PHYTASE FROM ISOLATE K46B USING RESPONSE SURFACE METHODOLOGY. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 13(1 (SPECIAL ISSUE)), 203-215. SID. https://sid.ir/paper/72174/en

Vancouver: Copy

ROCKY SALIMI K., HASHEMI M., SAFARI M.. OPTIMIZATION OF CATALYTIC ACTIVITY OF PHYTASE FROM ISOLATE K46B USING RESPONSE SURFACE METHODOLOGY. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2015;13(1 (SPECIAL ISSUE)):203-215. Available from: https://sid.ir/paper/72174/en

IEEE: Copy

K. ROCKY SALIMI, M. HASHEMI, and M. SAFARI, “OPTIMIZATION OF CATALYTIC ACTIVITY OF PHYTASE FROM ISOLATE K46B USING RESPONSE SURFACE METHODOLOGY,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 13, no. 1 (SPECIAL ISSUE), pp. 203–215, 2015, [Online]. Available: https://sid.ir/paper/72174/en

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