Fabrication of an amperometric aptasensor for determination of antibioticamount residues in milk

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MOHAMMAD RAZDARI A.; GHASEMI VARNAMKHASTI M.; ROSTAMI S.; ENSAFI A.A.; IZADI Z.; BONYADIAN M.

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Paper Information

Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2019 | Volume:16 | Issue:89 Page(s): 47-57

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Title

Fabrication of an amperometric aptasensor for determination of antibioticamount residues in milk

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Keywords

AptasensorQ1

Amperometric AntibioticQ1

NanomaterialQ2

Differential Pulse Voltammetry (DPV)Q1

Abstract

Quantitative detection of drug residues in animal food stuffs is very important. Excessive use of veterinarian veterinarians, like antibiotics are a serious threat to consumers, due to the residence of livestock products such as meat, milk, eggs. Rapid detection of antibiotics is essential by using an efficient, fast, affordable, and specific tool for risk reduction and food safety control. In the present study, an Aptasensor based on pencil graphite electrode modified with Nanomaterial including grapheme and gold, for rapid detection of tetracycline antibiotic was developed in milk samples. Cycle voltammetry and differential pulse voltammetry (DPV) techniques were used for response evaluation of Aptasensor. In order to modification the graphite pencil electrode, the scanrate (40 mV/s) and the number of cycles (10) and immobilization time of graphene (90 min) were optimized. Under optimum conditions, using differential pulse voltammetry technique was found to increase linearly in the range of 1 × 10-12 to 1 × 10-5 M, with increasing concentration (R² = 0. 985). The detection limit of the Aptasensor was found to be 1. 4× 10-13 M. A review of functional characteristics including repeatability, reproducibility, satability, and selectivity suggests acceptable performance for Aptasensor. Overall, the fabricated Aptasensor has efficiency required to detect tetracycline in milk samples.

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APA: Copy

MOHAMMAD RAZDARI, A., GHASEMI VARNAMKHASTI, M., ROSTAMI, S., ENSAFI, A.A., IZADI, Z., & BONYADIAN, M.. (2019). Fabrication of an amperometric aptasensor for determination of antibioticamount residues in milk. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 16(89 ), 47-57. SID. https://sid.ir/paper/72295/en

Vancouver: Copy

MOHAMMAD RAZDARI A., GHASEMI VARNAMKHASTI M., ROSTAMI S., ENSAFI A.A., IZADI Z., BONYADIAN M.. Fabrication of an amperometric aptasensor for determination of antibioticamount residues in milk. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2019;16(89 ):47-57. Available from: https://sid.ir/paper/72295/en

IEEE: Copy

A. MOHAMMAD RAZDARI, M. GHASEMI VARNAMKHASTI, S. ROSTAMI, A.A. ENSAFI, Z. IZADI, and M. BONYADIAN, “Fabrication of an amperometric aptasensor for determination of antibioticamount residues in milk,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 16, no. 89 , pp. 47–57, 2019, [Online]. Available: https://sid.ir/paper/72295/en

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