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Information Journal Paper

Title

Comparison of Epigenetic Modifier Genes in Bovine Adipose Tissue-Derived Stem Cell Based Embryos, as Donors, with In Vitro and Parthenogenesis Embryos

Pages

  149-157

Abstract

 Objective: Regarding that undifferentiated Mesenchymal Stem Cells, as donor cells, require less epigenetic reprogramming, possibility of using bovine adipose tissue-derived stem cells (BASCs) with low level of DNMTs and HDACs expression was evaluated. Materials and Methods: In this experimental study, we examined gene expression of epigenetic modifiers including DNA Methyltransferases (DNMT1, DNMT3A and DNMT3B) and Histone Deacetylases (HDAC1-3), as well as protein levels of histone H3 acetylation at lysine 9 (H3K9ac) and POU5F1 (also known as OCT4) at two stages of preimplantation development among in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) groups. Results: The results revealed that developmental competence of IVF embryos was higher than SCNT embryos (P<0. 05). In the PA and SCNT groups, DNMT1, HDAC2 and HDAC3 mRNA were overexpressed (P<0. 05), and proteins levels of H3K9ac and POU5F1 were reduced at 6-8 cells and blastocyst stages compared to IVF (P<0. 05). The mRNA expression of DNMT1 and HDAC1 and proteins levels of POU5F1 and H3K9ac were significantly different between SCNT and PA groups (P<0. 05) in both developmental stages (except HDAC1 in blastocyst stage). Conclusion: The SCNT embryos derived from BASCs have endured considerable nuclear reprogramming during early embryo development. Comparison of PA and SCNT blastocysts demonstrated that HDAC1 and DNMT1 may attribute to developmental competence variability of bovine embryos.

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    APA: Copy

    SALEHI, MOHAMMAD, ABOUHAMZEH, BEHESHTEH, HOSSEINI, AHMAD, Zare, Zohreh, & Bakhtari, Azizollah. (2020). Comparison of Epigenetic Modifier Genes in Bovine Adipose Tissue-Derived Stem Cell Based Embryos, as Donors, with In Vitro and Parthenogenesis Embryos. CELL JOURNAL (YAKHTEH), 22(2), 149-157. SID. https://sid.ir/paper/725116/en

    Vancouver: Copy

    SALEHI MOHAMMAD, ABOUHAMZEH BEHESHTEH, HOSSEINI AHMAD, Zare Zohreh, Bakhtari Azizollah. Comparison of Epigenetic Modifier Genes in Bovine Adipose Tissue-Derived Stem Cell Based Embryos, as Donors, with In Vitro and Parthenogenesis Embryos. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(2):149-157. Available from: https://sid.ir/paper/725116/en

    IEEE: Copy

    MOHAMMAD SALEHI, BEHESHTEH ABOUHAMZEH, AHMAD HOSSEINI, Zohreh Zare, and Azizollah Bakhtari, “Comparison of Epigenetic Modifier Genes in Bovine Adipose Tissue-Derived Stem Cell Based Embryos, as Donors, with In Vitro and Parthenogenesis Embryos,” CELL JOURNAL (YAKHTEH), vol. 22, no. 2, pp. 149–157, 2020, [Online]. Available: https://sid.ir/paper/725116/en

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