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Information Journal Paper

Title

A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery

Pages

  1109-1118

Abstract

 Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids. The first Reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-1 was assayed by this system and was compared to the results obtained by other methods. Results: HTLV-1 reporter cell line was found to produce high level of Luciferase when co-cultured with MT-2 and Hut-102 cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-1 can reduce Luciferase expression of the cell when co-cultured with MT-2 and Hut-102 comparable to the ELISA (R=0. 932, P-value =0. 002). In addition, the results revealed the superiority of the present system over the molecular methods. Conclusion: The results demonstrated that the Biological assay system is a beneficial tool for the medium-throughput anti-HTLV-1 Drug screening and inhibitory effect.

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    • Cite

    APA: Copy

    FATTAHI ABDIZADEH, MOJTABA, MAKVANDI, MANOOCHEHR, SAMARBAFZADEH, ALIREZA, & AZADMANESH, KAYHAN. (2017). A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(10), 1109-1118. SID. https://sid.ir/paper/726655/en

    Vancouver: Copy

    FATTAHI ABDIZADEH MOJTABA, MAKVANDI MANOOCHEHR, SAMARBAFZADEH ALIREZA, AZADMANESH KAYHAN. A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(10):1109-1118. Available from: https://sid.ir/paper/726655/en

    IEEE: Copy

    MOJTABA FATTAHI ABDIZADEH, MANOOCHEHR MAKVANDI, ALIREZA SAMARBAFZADEH, and KAYHAN AZADMANESH, “A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 10, pp. 1109–1118, 2017, [Online]. Available: https://sid.ir/paper/726655/en

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