The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds

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Shafieian Reihaneh; MOGHADDAM MATIN MARYAM; RAHPEYMA AMIN; FAZEL ALIREZA; Salari Sedigh Hamideh; SADR NABAVI ARIANE; Hassanzadeh Halimeh; EBRAHIMZADEH BIDESKAN ALIREZA

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Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:10 Page(s): 1131-1140

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Title

The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds

Author(s)

Keywords

Adipose tissue

Bone

Dog

Osteogenesis

Stem cells

Tissue engineering

Abstract

Objective(s): Autologous Bone transplantation known as the “ gold standard” to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the Osteogenesis ability of human adipose-derived mesenchymal Stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular Bones of 5 mongrel Dogs and divided randomly into the following groups: I-autologous crushed Bone, II-no filling material, III-HA/TCP and PRP, IV-PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to Bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively. Results: In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<0. 05). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable Bone formation in osseous defects (P<0. 05). It was obviously shown that hAdMSCs were incorporated into the newly-formed Bone. Conclusion: Based on this study, application of Stem cells could offer a helpful therapeutic tool in Bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed Bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration.

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APA: Copy

Shafieian, Reihaneh, MOGHADDAM MATIN, MARYAM, RAHPEYMA, AMIN, FAZEL, ALIREZA, Salari Sedigh, Hamideh, SADR NABAVI, ARIANE, Hassanzadeh, Halimeh, & EBRAHIMZADEH BIDESKAN, ALIREZA. (2017). The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(10), 1131-1140. SID. https://sid.ir/paper/726656/en

Vancouver: Copy

Shafieian Reihaneh, MOGHADDAM MATIN MARYAM, RAHPEYMA AMIN, FAZEL ALIREZA, Salari Sedigh Hamideh, SADR NABAVI ARIANE, Hassanzadeh Halimeh, EBRAHIMZADEH BIDESKAN ALIREZA. The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(10):1131-1140. Available from: https://sid.ir/paper/726656/en

IEEE: Copy

Reihaneh Shafieian, MARYAM MOGHADDAM MATIN, AMIN RAHPEYMA, ALIREZA FAZEL, Hamideh Salari Sedigh, ARIANE SADR NABAVI, Halimeh Hassanzadeh, and ALIREZA EBRAHIMZADEH BIDESKAN, “The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 10, pp. 1131–1140, 2017, [Online]. Available: https://sid.ir/paper/726656/en

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