Hsa-miR-587 Regulates TGFβ /SMAD Signaling and Promotes Cell Cycle Progression

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Jahangirimoez Mahnaz; Medlej Abdallah; TAVALAEI MAHMOUD; Soltani Bahram Mohammad

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2020 | Volume:22 | Issue:2 Page(s): 158-164

Download Full-Text

View:

319

Download:

697

Cites:

Information Journal Paper

Title

Hsa-miR-587 Regulates TGFβ /SMAD Signaling and Promotes Cell Cycle Progression

Author(s)

Jahangirimoez Mahnaz | Medlej Abdallah | TAVALAEI MAHMOUD | Soltani Bahram Mohammad | Issue Writer Certificate

Keywords

Cancer

Cell Cycle

miR-587

TGFβ/SMAD Signaling

Abstract

Objective: Transforming growth factor beta/single mothers against decapentaplegic (TGFβ /SMAD) signaling pathway plays important roles in various biological processes. It acts as a tumor suppressor during the early stages of Cancer progression. Discovering the regulators of this pathway provides important options for therapeutic strategies. Here, we searched for candidate microRNAs (miRNAs) that potentially target the critical components of the TGFβ signaling pathway. Materials and Methods: In the current experimental study, we first predicted miRNAs that target TGFβ components using a bioinformatics software. After that, quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-587, TGFBR2, SMAD4, p21, CCND1 and c-MYC genes in transfected HEK293T and HCT116 cells. Dual Luciferase assay was performed to analyze the interactions between miRNAs and the target genes. Propidium iodide flow cytometry was used to determine Cell Cycle progression in HEK293T and HCT116 cells under hsa-miR-587 (miR-587) overexpression circumstances. Results: Multiple miRNA responsive elements (MREs) were predicted for miR-587 within the 3’ UTRs of the TGFBR2 and SMAD4 genes. Overexpression of miR-587 in HEK293T and HCT116 cells resulted in downregulation of TGFBR2 and SMAD4 genes. In addition, a downstream target gene of TGFβ /SMAD signaling, P21, was significantly downregulated in the HCT116 cells overexpressing miR-587. Dual luciferase assay analysis provided evidence that there is a direct interaction between miR-587 and the 3’ UTR sequences of TGFBR2 and SMAD4 genes. Moreover, miR-587 overexpression in HEK293T and HCT116 cells resulted in reducing the SubG1 cell populations in both cell lines, as detected by flow cytometry. Conclusion: Altogether, our data revealed an important role for miR-587 in regulating TGFβ /SMAD signaling and promoting Cell Cycle progression. These characteristics suggest that miR-587 is an important candidate for Cancer therapy research.

Cites

No record.

References

Cite

APA: Copy

Jahangirimoez, Mahnaz, Medlej, Abdallah, TAVALAEI, MAHMOUD, & Soltani, Bahram Mohammad. (2020). Hsa-miR-587 Regulates TGFβ /SMAD Signaling and Promotes Cell Cycle Progression. CELL JOURNAL (YAKHTEH), 22(2), 158-164. SID. https://sid.ir/paper/727368/en

Vancouver: Copy

Jahangirimoez Mahnaz, Medlej Abdallah, TAVALAEI MAHMOUD, Soltani Bahram Mohammad. Hsa-miR-587 Regulates TGFβ /SMAD Signaling and Promotes Cell Cycle Progression. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(2):158-164. Available from: https://sid.ir/paper/727368/en

IEEE: Copy

Mahnaz Jahangirimoez, Abdallah Medlej, MAHMOUD TAVALAEI, and Bahram Mohammad Soltani, “Hsa-miR-587 Regulates TGFβ /SMAD Signaling and Promotes Cell Cycle Progression,” CELL JOURNAL (YAKHTEH), vol. 22, no. 2, pp. 158–164, 2020, [Online]. Available: https://sid.ir/paper/727368/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops