INCREASED TOXICITY OF CHEMOTHERAPEUTIC DRUGS BY ALL-TRANS RETINOIC ACID IN CD44 CELLS

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ABBASI A.; MAZANI M.; NAJAFZADEH N.; AMANI M.; SHEYKHKANLOOY MILAN H.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2016 | Volume:18 | Issue:3 Page(s): 25-32

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Title

INCREASED TOXICITY OF CHEMOTHERAPEUTIC DRUGS BY ALL-TRANS RETINOIC ACID IN CD44 CELLS

Author(s)

Keywords

KYSE-30 CELL LINEQ2

RETINOIC ACIDQ2

CISPLATINQ2

5-FLUOROURACILQ1

CD44Q2

CHEMOTHERAPYQ2

Abstract

BACKGROUND AND OBJECTIVE: In recent studies, undifferentiated CD44 cells have been introduced as the major cause of chemotherapeutic drug resistance in esophageal cancer. In this study, we aimed to evaluate the effects of all-trans RETINOIC ACID on reducing chemotherapeutic drug resistance and improving the associated toxic effects.METHODS: In this clinical study, CD44+ and CD44- cells were separated from KYSE-30 CELL LINE, using magnetic-activated cell sorting (MACS) method. The cytotoxic effects of RETINOIC ACID treatment, combined with CISPLATIN and 5-FLUOROURACIL, were separately evaluated in two cell groups, i.e., CD44+ and CD44-. Cytotoxicity was determined by identifying cellular metabolic activity, acridine orange/ethidium bromide staining, and flow cytometry.FINDINGS: In this study, CD44 marker was expressed in 6.25% of the cell population in KYSE-30 CELL LINE. The results of flow cytometry revealed that treatment with a combination of RETINOIC ACID and chemotherapeutic drugs could improve cell cycle arrest in CD44+ cells (p<0.05), unlike CD44- cells. Determination of cellular metabolic activity, increased cell apoptosis along with decreased half maximal inhibitory concentration (IC50), and acridine orange/ethidium bromide staining were indicative of the increased percentage of primary and secondary apoptotic CD44+ cells. However, in CD44- cells, these effects were only observed by using a combination of RETINOIC ACID and CISPLATIN (p<0.05).CONCLUSION: The present results showed that all-trans RETINOIC ACID could increase the toxicity of CISPLATIN and 5-FLUOROURACIL in CD44+ cells.

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APA: Copy

ABBASI, A., MAZANI, M., NAJAFZADEH, N., AMANI, M., & SHEYKHKANLOOY MILAN, H.. (2016). INCREASED TOXICITY OF CHEMOTHERAPEUTIC DRUGS BY ALL-TRANS RETINOIC ACID IN CD44 CELLS. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 18(3), 25-32. SID. https://sid.ir/paper/73397/en

Vancouver: Copy

ABBASI A., MAZANI M., NAJAFZADEH N., AMANI M., SHEYKHKANLOOY MILAN H.. INCREASED TOXICITY OF CHEMOTHERAPEUTIC DRUGS BY ALL-TRANS RETINOIC ACID IN CD44 CELLS. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2016;18(3):25-32. Available from: https://sid.ir/paper/73397/en

IEEE: Copy

A. ABBASI, M. MAZANI, N. NAJAFZADEH, M. AMANI, and H. SHEYKHKANLOOY MILAN, “INCREASED TOXICITY OF CHEMOTHERAPEUTIC DRUGS BY ALL-TRANS RETINOIC ACID IN CD44 CELLS,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 18, no. 3, pp. 25–32, 2016, [Online]. Available: https://sid.ir/paper/73397/en

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