Expression of recombinant G-CSF receptor domains and their inhibitory role on G-CSF function

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Bakherad Hamid; SETAYESH NEDA; MOUSAVI GARGARI SEYED LATIF; EBRAHIMIZADEH WALEAD; Mavandadnejad Faranak; FAGHFURI ELNAZ; EBRAHIMI SOHEILA; HEIAT MOHAMMAD; Shahpari Mona; SEPEHRIZADEH ZARGHAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: RESEARCH IN PHARMACEUTICAL SCIENCES (RPS) Year:2020 | Volume:15 | Issue:4 Page(s): 381-389

Download Full-Text

View:

151

Download:

Cites:

Information Journal Paper

Title

Expression of recombinant G-CSF receptor domains and their inhibitory role on G-CSF function

Author(s)

Keywords

CSF antagonist;G

CSF

R domains;NFS60

Abstract

Background and purpose: Granulocyte colony-stimulating factor (G-CSF) is routinely used in combination with chemotherapy to battle neutropenia. However, studies suggest that this chemokine may increase the risk of metastasis and malignancy in many cancers. To counteract the adverse effects of G-CSF in cancer, antibodies have been used to block its action. However, antibodies are large and complex molecules which makes their production expensive. Thus in this study, we aim to construct different structure variants of the G-CSF receptor containing different domains and select the best variant that prevents the adverse actions of this chemokine. These novel structures are smaller than antibodies and easier to produce. Experimental approach: Different domains of the G-CSF receptor were designed and cloned into the pET28a expression vector. These recombinant receptor subunits were then expressed in Escherichia coli and purified using standard affinity chromatography techniques. Interaction of recombinant receptor subunits with G-CSF was assessed using enzyme-linked immunosorbent assay and NFS60 cells. Findings / Results: Two recombinant receptor subunits containing D1 + D2 + D3 domains and D2 domain showed the strongest inhibitory activity to G-CSF. Conclusion and implications: These novel recombinant receptor variants could be candidates for further studies in the development of novel therapeutics.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

Bakherad, Hamid, SETAYESH, NEDA, MOUSAVI GARGARI, SEYED LATIF, EBRAHIMIZADEH, WALEAD, Mavandadnejad, Faranak, FAGHFURI, ELNAZ, EBRAHIMI, SOHEILA, HEIAT, MOHAMMAD, Shahpari, Mona, & SEPEHRIZADEH, ZARGHAM. (2020). Expression of recombinant G-CSF receptor domains and their inhibitory role on G-CSF function. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), 15(4), 381-389. SID. https://sid.ir/paper/738989/en

Vancouver: Copy

Bakherad Hamid, SETAYESH NEDA, MOUSAVI GARGARI SEYED LATIF, EBRAHIMIZADEH WALEAD, Mavandadnejad Faranak, FAGHFURI ELNAZ, EBRAHIMI SOHEILA, HEIAT MOHAMMAD, Shahpari Mona, SEPEHRIZADEH ZARGHAM. Expression of recombinant G-CSF receptor domains and their inhibitory role on G-CSF function. RESEARCH IN PHARMACEUTICAL SCIENCES (RPS)[Internet]. 2020;15(4):381-389. Available from: https://sid.ir/paper/738989/en

IEEE: Copy

Hamid Bakherad, NEDA SETAYESH, SEYED LATIF MOUSAVI GARGARI, WALEAD EBRAHIMIZADEH, Faranak Mavandadnejad, ELNAZ FAGHFURI, SOHEILA EBRAHIMI, MOHAMMAD HEIAT, Mona Shahpari, and ZARGHAM SEPEHRIZADEH, “Expression of recombinant G-CSF receptor domains and their inhibitory role on G-CSF function,” RESEARCH IN PHARMACEUTICAL SCIENCES (RPS), vol. 15, no. 4, pp. 381–389, 2020, [Online]. Available: https://sid.ir/paper/738989/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops