PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

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DARVISHI MARZIEH; GHASEMI HAMIDABADI HATEF; SAHAB NEGAH SAJAD; MOAYERI ARDESHIR; TIRAIHI TAKI; MIRNAJAFI ZADEH JAVAD; JAHANBAZI JAHAN ABAD ALI; SHOJAEI AMIR

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Journal: Iranian Journal of Basic Medical Sciences Year:2020 | Volume:23 | Issue:4 Page(s): 431-438

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Title

PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

Author(s)

Keywords

Motor neuron-like cells

Nanoscaffolds

Proliferation

Stem cell therapy

Three-dimension culture

Tissue engineering

Abstract

Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in Stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and Tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional Motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared Motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, Proliferation, and functional properties on two-dimensional with threedimensional culture systems. Neural differentiation was analyzed by immunocytochemistry for immature (Islet1) and mature (HB9, ChAT, and synaptophysin) motor neuron markers. Results: Our results indicated that the three-dimensional environment exhibited an increase in the number of Islet1. In contrast, two-dimensional culture system resulted in more homeobox gene (HB9), Choline Acetyltransferase (ChAT), and synaptophysin positive cells. The results of this investigation showed that Proliferation and adhesion of Motor neuron-like cells significantly increased in threedimensional compared with two-dimensional environments. Conclusion: The findings of this study suggested that three-dimension may create a proliferative niche for Motor neuron-like cells. Overall, this study strengthens the idea that three-dimensional culture may mimic neural stem cell environment for neural tissue regeneration.

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APA: Copy

DARVISHI, MARZIEH, GHASEMI HAMIDABADI, HATEF, SAHAB NEGAH, SAJAD, MOAYERI, ARDESHIR, TIRAIHI, TAKI, MIRNAJAFI ZADEH, JAVAD, JAHANBAZI JAHAN ABAD, ALI, & SHOJAEI, AMIR. (2020). PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 23(4), 431-438. SID. https://sid.ir/paper/745738/en

Vancouver: Copy

DARVISHI MARZIEH, GHASEMI HAMIDABADI HATEF, SAHAB NEGAH SAJAD, MOAYERI ARDESHIR, TIRAIHI TAKI, MIRNAJAFI ZADEH JAVAD, JAHANBAZI JAHAN ABAD ALI, SHOJAEI AMIR. PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2020;23(4):431-438. Available from: https://sid.ir/paper/745738/en

IEEE: Copy

MARZIEH DARVISHI, HATEF GHASEMI HAMIDABADI, SAJAD SAHAB NEGAH, ARDESHIR MOAYERI, TAKI TIRAIHI, JAVAD MIRNAJAFI ZADEH, ALI JAHANBAZI JAHAN ABAD, and AMIR SHOJAEI, “PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 23, no. 4, pp. 431–438, 2020, [Online]. Available: https://sid.ir/paper/745738/en

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