AN EVIDENCE FOR A POTASSIUM CHANNEL IN ENDOPLASMIC RETICULUM BASED ON SINGLE CHANNEL RECORDING IN BILAYER LIPID MEMBRANE

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SEPEHRI HAMID; ELIASI AFSANEH; SAGHIRI R.

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Journal: Physiology and Pharmacology Year:2006 | Volume:10 | Issue:2 Page(s): 165-171

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Title

AN EVIDENCE FOR A POTASSIUM CHANNEL IN ENDOPLASMIC RETICULUM BASED ON SINGLE CHANNEL RECORDING IN BILAYER LIPID MEMBRANE

Author(s)

SEPEHRI HAMID | ELIASI AFSANEH | SAGHIRI R. | Issue Writer Certificate

Keywords

HEPATOCYTEQ3

ENDOPLASMIC RETICULUMQ4

BLMQ3

SINGLE CHANNEL RECORDINGQ3

K+ CHANNELQ3

Abstract

Introduction Numerous studies have demonstrated the presence of potassium selective channels in membranes of internal organelles. These channels are essential to a large variety of cellular processes including intracellular Ca2+ signaling, protein recycling, charge neutralization and cell protection. In contrast to the sarcoplasmicreticulum where potassium channels have been clearly identified, there is little evidence for K+ selective cannels in RER of HEPATOCYTEs. The aim of this study is to find an evidence for presence of potassium channel in ENDOPLASMIC RETICULUM and considering the pharmacological and biophysical properties of this channel. Methods: HEPATOCYTEs RER vesicles were isolatedby homogenizing rat liver followed by several centrifuging steps and then incorporated into the bilayer lipid membrane (BLM). The BLM was formed by painting phosphatidylcholine across the 350 mm aperture seperating two chambers (cis chamber containing 200 mMKCl and trans chamber containing 50 mM KCI). SINGLE CHANNEL RECORDINGs were used to indicate the presence of K+ channels.Results: SINGLE CHANNEL RECORDINGs revealed the existence of a cation selective channel with high permeability to K+ and 599 pS conductance. The current-voltage relation was linear. The open probability was strongly voltage dependent, showing higher values at positive voltages and lower at negative voltages. A sub conductance state about 60 % of fully open state was observed at all voltages. The cationic channel showed an inhibition by 4- aminnopyridine (non specific K+ channel blocker) Conclusions: In this study we have established an evidence for existence of large conductance K+ channel in the HEPATOCYTE ER vesicles.

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APA: Copy

SEPEHRI, HAMID, ELIASI, AFSANEH, & SAGHIRI, R.. (2006). AN EVIDENCE FOR A POTASSIUM CHANNEL IN ENDOPLASMIC RETICULUM BASED ON SINGLE CHANNEL RECORDING IN BILAYER LIPID MEMBRANE . PHYSIOLOGY AND PHARMACOLOGY, 10(2), 165-171. SID. https://sid.ir/paper/74894/en

Vancouver: Copy

SEPEHRI HAMID, ELIASI AFSANEH, SAGHIRI R.. AN EVIDENCE FOR A POTASSIUM CHANNEL IN ENDOPLASMIC RETICULUM BASED ON SINGLE CHANNEL RECORDING IN BILAYER LIPID MEMBRANE . PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2006;10(2):165-171. Available from: https://sid.ir/paper/74894/en

IEEE: Copy

HAMID SEPEHRI, AFSANEH ELIASI, and R. SAGHIRI, “AN EVIDENCE FOR A POTASSIUM CHANNEL IN ENDOPLASMIC RETICULUM BASED ON SINGLE CHANNEL RECORDING IN BILAYER LIPID MEMBRANE ,” PHYSIOLOGY AND PHARMACOLOGY, vol. 10, no. 2, pp. 165–171, 2006, [Online]. Available: https://sid.ir/paper/74894/en

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