THE EFFECT OF CO-ADMINISTRATION OF VITAMINS E AND C ON GENTAMICIN NEPHROTOXICITY IN RAT PERFUSED KIDNEY

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KHASTAR M.; KADKHODAEI M.; FAGHIHI M.; SHAMS S.; GHAZNAVI P.; ZAHMATKESH M.

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Journal: Physiology and Pharmacology Year:2004 | Volume:8 | Issue:1 Page(s): 93-100

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Title

THE EFFECT OF CO-ADMINISTRATION OF VITAMINS E AND C ON GENTAMICIN NEPHROTOXICITY IN RAT PERFUSED KIDNEY

Author(s)

Keywords

GENTAMICINQ3

VITAMIN EQ3

VITAMIN CQ3

ISOLATED KIDNEYQ4

Abstract

GENTAMICIN (GM) is an effective antibiotic against severe Gram-negative bacteria.However it can produce nephrotoxicity in human. Reactive oxygen species have been proposed as the causative factors of its renal side effects. This study was performed on the protective role of antioxidant vitamins against GM-mediated nephropathy in an in situ model of isolated rat kidney.For this purpose, male Sprague-Dawley rats were randomly assigned to one of the following groups of seven rats: Group 1 (control) was tyrode-perfused kidneys. Group 2 (GM), in which 200 µg/ml of GENTAMICIN was added to the perfusate. Group 3 (GM + Vit C), the same as group 2 but VITAMIN C (200 mg/L) was added to the drinking water for 3 days and 100 mg/L to the perfusate. Group 4 (GM + Vit E), the same as group 2 but VITAMIN E (100 mg/100 g BW, i.p.) was injected 12 h before experiment. Group 5 (GM + Vit C + Vit E) the same as group 2 but vitamins E and C were co-administered (the same as Groups 3 and 4). To compare the groups, urinary lactate dehydrogenase (LDH), N-acetyl-B-D-glucoseaminidase (NAG), alkaline phosphates (ALP) activities, inulin clearance (glomerular filtration rate, GFR) and renal tissue glutathione (GSH) contents were measured. The results showed that GENTAMICIN causes a significant nephrotoxicity as demonstrated by an increase in urinary LDH, NAG, and ALP. In addition, a decline in GSH and a marked decrease in GFR were observed as compared to controls. On the other hand, VITAMIN C inhibited the GENTAMICIN-induced increase in urinary enzymes, but did not show a significant effect on the GSH and GFR. In. this respect, VITAMIN E not only inhibited GENTAMICIN-induced increase in urinary enzymes, but also prevented the GENTAMICIN-induced GSH decline without a significant improvement regarding GFR. Furthermore, co-administration of vitamins C and E significantly prevented the GM-induced nephrotoxicity as demonstrated by preservation of GFR and GSP levels and prevention of an increase in urinary enzyme activities. Therefore, it is concluded that co-administration of moderate doses of vitamins C and E has beneficial effects on renal preservation and protection in gentainicin-induced nephrotoxicity.

Cites

RENAL OXIDATIVE STRESS STATUS AND HISTOLOGY IN GENTAMICIN NEPHROTOXICITY: THE EFFECTS OF ANTIOXIDANT VITAMINS

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APA: Copy

KHASTAR, M., KADKHODAEI, M., FAGHIHI, M., SHAMS, S., GHAZNAVI, P., & ZAHMATKESH, M.. (2004). THE EFFECT OF CO-ADMINISTRATION OF VITAMINS E AND C ON GENTAMICIN NEPHROTOXICITY IN RAT PERFUSED KIDNEY. PHYSIOLOGY AND PHARMACOLOGY, 8(1), 93-100. SID. https://sid.ir/paper/75275/en

Vancouver: Copy

KHASTAR M., KADKHODAEI M., FAGHIHI M., SHAMS S., GHAZNAVI P., ZAHMATKESH M.. THE EFFECT OF CO-ADMINISTRATION OF VITAMINS E AND C ON GENTAMICIN NEPHROTOXICITY IN RAT PERFUSED KIDNEY. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2004;8(1):93-100. Available from: https://sid.ir/paper/75275/en

IEEE: Copy

M. KHASTAR, M. KADKHODAEI, M. FAGHIHI, S. SHAMS, P. GHAZNAVI, and M. ZAHMATKESH, “THE EFFECT OF CO-ADMINISTRATION OF VITAMINS E AND C ON GENTAMICIN NEPHROTOXICITY IN RAT PERFUSED KIDNEY,” PHYSIOLOGY AND PHARMACOLOGY, vol. 8, no. 1, pp. 93–100, 2004, [Online]. Available: https://sid.ir/paper/75275/en

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