THE EFFECT OF RENAL ISCHEMIA-REPERFUSION ON HYDROXYL RADICAL PRODUCTION AND PLASMA CONCENTRATION OF COPPER AND ZINC: AN IN VIVO STUDY

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SAFARI M.SH.; FAGHIHI M.; PARVIZ M.; KADKHODAEI MEHRI; FARZAMI B.; SHAMS S.

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Journal: Physiology and Pharmacology Year:2004 | Volume:8 | Issue:1 Page(s): 61-70

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Title

THE EFFECT OF RENAL ISCHEMIA-REPERFUSION ON HYDROXYL RADICAL PRODUCTION AND PLASMA CONCENTRATION OF COPPER AND ZINC: AN IN VIVO STUDY

Author(s)

Keywords

KIDNEYQ4

ISCHEMIA-REPERFUSIONQ2

HYDROXYL RADICALQ3

CUQ3

ZNQ3

Abstract

Reestablishment of blood perfusion to ischemic tissues paradoxically results in further tissue injury, which is called ischemic-reperfusion (IR) injury. Injurious factors are reactive oxygen species (ROS) and among them HYDROXYL RADICAL (OH) is the most dangerous. Aromatic cycle trapping of HYDROXYL RADICAL and detection of hydroxylated metabolites. With high performance liquid chromatography and electrochemical detectors (HPLC-ECD) has been known to be the most sensitive method of ROS detection. Efficacy assessment of intravenous injection of aromatic cycles via this method and estimation of the effect of metabolic pathways in production of these metabolites were the most important goals of this research study. Since redox active metals including Fe and CU and non-redox active metals like ZN have important roles in production or inhibition of ROS production, therefore, assessment of plasma changes of CU and ZN concentration during reperfusion was another purpose of this research. For this purpose, bilateral IR of rat KIDNEY pedicles was conducted. Rate of salicylate trapping of OH and production of dihydroxy benzoic acids (DHBA) as indices of OH production was measured. Therefore, plasma concentration of CU and ZN were measured using atomic absorption spectrophotometer 30 and 90 minutes after reperfusion. Renal injury was confirmed by measurements of plasma creatinine concentration before and 24 hours after reperfusion. Data analysis using ANOVA test indicated that renal IR significantly increased OH production after reperfusion and OH causes renal nephrotubular injury.This research showed that intravenous injection of sodium salicylate and measurement of its hydroxylated metabolite can be considered as a reliable method for measurement of HYDROXYL RADICAL production during reperfusion. The time-dependent pattern as observed in this research together with estimation of metabolic production rate of 2,5 DHBA may induce the idea for using it for the measurement of HYDROXYL RADICAL production. Also, this study implied that reperfusion after 1 hour bilateral renal ischemia does not change plasma ZN and CU concentration.

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APA: Copy

SAFARI, M.SH., FAGHIHI, M., PARVIZ, M., KADKHODAEI, MEHRI, FARZAMI, B., & SHAMS, S.. (2004). THE EFFECT OF RENAL ISCHEMIA-REPERFUSION ON HYDROXYL RADICAL PRODUCTION AND PLASMA CONCENTRATION OF COPPER AND ZINC: AN IN VIVO STUDY. PHYSIOLOGY AND PHARMACOLOGY, 8(1), 61-70. SID. https://sid.ir/paper/75276/en

Vancouver: Copy

SAFARI M.SH., FAGHIHI M., PARVIZ M., KADKHODAEI MEHRI, FARZAMI B., SHAMS S.. THE EFFECT OF RENAL ISCHEMIA-REPERFUSION ON HYDROXYL RADICAL PRODUCTION AND PLASMA CONCENTRATION OF COPPER AND ZINC: AN IN VIVO STUDY. PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2004;8(1):61-70. Available from: https://sid.ir/paper/75276/en

IEEE: Copy

M.SH. SAFARI, M. FAGHIHI, M. PARVIZ, MEHRI KADKHODAEI, B. FARZAMI, and S. SHAMS, “THE EFFECT OF RENAL ISCHEMIA-REPERFUSION ON HYDROXYL RADICAL PRODUCTION AND PLASMA CONCENTRATION OF COPPER AND ZINC: AN IN VIVO STUDY,” PHYSIOLOGY AND PHARMACOLOGY, vol. 8, no. 1, pp. 61–70, 2004, [Online]. Available: https://sid.ir/paper/75276/en

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