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Information Journal Paper

Title

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Pages

  0-0

Abstract

 Background: Cosmos bipinnatus is an important medicinal plant with antioxidative, antigenotoxic, anti-infl ammatory, and antiproliferative eff ects on several cancer cell lines. It has great potential for development as a promising cancer chemo-preventive agent. Hairy Root-based culture technology is a new sustainable production platform for producing specifi c pharmaceutical secondary metabolites. Objectives: The current study developed and introduced a reliable transformation system for C. bipinnatus by optimization of aspects important in transformation frequency using Agrobacterium rhizogenes. Methods: Five bacterial strains, including ATCC 15834, ATCC 31798, A7, MAFF-02-10266, and MSU440, 2 explant types (leaf and stem), and 2 co-cultivation media (full MS and ½ MS) were examined. Genomic DNA was extracted using a modifi ed CTAB protocol from putative transgenic root lines and the control root. Transgenic Hairy Root lines were approved by means of Polymerase Chain Reaction (PCR) using specifi c rolB gene primers. Results: The highest ratio of genetically transformed root induction was found from leaf explants using A. rhisogenes strains ATCC15834 and MSU440 (72% to 73%). When ½ MS medium was used as a co-cultivation medium, a signifi cant increase in transformation frequency (84%) was observed. Conclusions: The MSU440 Agrobacterium strain and ½ MS co-cultivation medium could signifi cantly improve genetic transformation effi ciency for establishment of Hairy Root-based cultures for C. bipinnatus.

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    APA: Copy

    Jaberi, Mehdi, SHARAFI, ALI, Sharafi, Ata Allah, AZADI, PEJMAN, KHEIRI MANJILI, HAMIDREZA, DANAFAR, HOSSEIN, & Ahmadnia, Alireza. (2018). Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus. JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS, 13(1), 0-0. SID. https://sid.ir/paper/753318/en

    Vancouver: Copy

    Jaberi Mehdi, SHARAFI ALI, Sharafi Ata Allah, AZADI PEJMAN, KHEIRI MANJILI HAMIDREZA, DANAFAR HOSSEIN, Ahmadnia Alireza. Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus. JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS[Internet]. 2018;13(1):0-0. Available from: https://sid.ir/paper/753318/en

    IEEE: Copy

    Mehdi Jaberi, ALI SHARAFI, Ata Allah Sharafi, PEJMAN AZADI, HAMIDREZA KHEIRI MANJILI, HOSSEIN DANAFAR, and Alireza Ahmadnia, “Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus,” JUNDISHAPUR JOURNAL OF NATURAL PHARMACEUTICAL PRODUCTS, vol. 13, no. 1, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/753318/en

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