مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

119
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

93
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Immunological Responses against HER2-targeted Idarubicin-ZHER2 Conjugate in BALB/c Mice

Pages

  501-510

Abstract

 Targeting of cancerous cells with a high level of Human epidermal growth factor receptor 2 (HER2) expressions by drug immunoconjugates is a new approach for specific delivery of chemotherapeutic agents. Our previous work indicated that Idarubicin-ZHER2 affibody conjugate has a great potential for the treatment of HER2-overexpressing malignant cell lines but possible induced Immune response against constructed conjugate was not addressed. In the current study, the possibility of induction of humoral and cellular Immune responses against Idarubicin-ZHER2 affibody conjugate in BALB/c mice was investigated. For assessment of the induced Immune response, prepared and qualified Idarubicin-ZHER2 affibody conjugate was administrated intravenously to BALB/c mice and the induced cellular Immune response was evaluated by measuring secretion levels of interferon gamma (IFN-γ ) and interleukin 10 (IL-10) Cytokines by the splenocytes. Humoral response of treated mice was also assessed by measuring total Immunoglobulin G (IgG) titer in mice sera. The obtained results showed that Idarubicin-ZHER2 affibody conjugate at any examined concentrations could not induce secretion of IFN-γ as a pro-inflammatory Cytokine. A mild increase in the level of regulatory IL-10 Cytokine was seen in the treated mice although no dose dependency in the level of IL-10 production was observed. Furthermore, results showed that idarubicin-ZHER2 conjugate could not induce IgG production in the treated mice. Based on these findings, the idarubicin-ZHER2 conjugate can be considered as a candidate for the development of new therapeutics against HER2-overexpressing cancers although further in vivo studies are needed.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Siavoshinia, Leila, JAMALAN, MOSTAFA, Zeinali, Majid, & MOHAMMADZADEH, GHORBAN. (2019). Immunological Responses against HER2-targeted Idarubicin-ZHER2 Conjugate in BALB/c Mice. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 18(5), 501-510. SID. https://sid.ir/paper/758416/en

    Vancouver: Copy

    Siavoshinia Leila, JAMALAN MOSTAFA, Zeinali Majid, MOHAMMADZADEH GHORBAN. Immunological Responses against HER2-targeted Idarubicin-ZHER2 Conjugate in BALB/c Mice. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2019;18(5):501-510. Available from: https://sid.ir/paper/758416/en

    IEEE: Copy

    Leila Siavoshinia, MOSTAFA JAMALAN, Majid Zeinali, and GHORBAN MOHAMMADZADEH, “Immunological Responses against HER2-targeted Idarubicin-ZHER2 Conjugate in BALB/c Mice,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 18, no. 5, pp. 501–510, 2019, [Online]. Available: https://sid.ir/paper/758416/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button