Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor-A

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KHAKI MOHSEN; SALMANIAN ALI HATEF; ABTAHI HAMID; GANJI ALI; MOSAYEBI GHASEM

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Journal: REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Year:2018 | Volume:6 | Issue:2 Page(s): 144-150

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Title

Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor-A

Author(s)

Keywords

Cell differentiation

Endothelial cell

Mesenchymal stem cell

Vascular Endothelial Growth Factor A

Abstract

Background: Vascular endothelial growth factor-A (VEGF-A), an Endothelial cell-specific mitogen produced by various cell types, plays important roles in Cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of Mesenchymal stem cells (MSCs) to Endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21 pLysS competent cells with the pET32a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting. Mesenchymal stem Cell differentiation to ECs in the presence of VEGF-A was evaluated by flow cytometry and fluorescence microscopy. Results: Recombinant VEGF-A was produced in E. coli BL21 (DE3) cells at 0. 8 mg/mL concentration. Expression of CD31 and CD 144 was significantly greater, while expression of CD90, CD73, and CD44 was significantly less, in MSCs treated with our recombinant VEGF-A than in those treated with the commercial protein (p < 0. 05). Conclusions: Recombinant VEGF-A expressed in a prokaryotic system can induce MSCs differentiation to ECs and can be used in research and likely therapeutic applications.

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ارزیابی تاثیر مواد افزودنی شیمیایی در تاخوردگی مجدد فاکتور رشد اندوتلیال عروقی نوترکیب

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APA: Copy

KHAKI, MOHSEN, SALMANIAN, ALI HATEF, ABTAHI, HAMID, GANJI, ALI, & MOSAYEBI, GHASEM. (2018). Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor-A. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 6(2), 144-150. SID. https://sid.ir/paper/763847/en

Vancouver: Copy

KHAKI MOHSEN, SALMANIAN ALI HATEF, ABTAHI HAMID, GANJI ALI, MOSAYEBI GHASEM. Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor-A. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2018;6(2):144-150. Available from: https://sid.ir/paper/763847/en

IEEE: Copy

MOHSEN KHAKI, ALI HATEF SALMANIAN, HAMID ABTAHI, ALI GANJI, and GHASEM MOSAYEBI, “Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor-A,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 6, no. 2, pp. 144–150, 2018, [Online]. Available: https://sid.ir/paper/763847/en

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